The efficiency and linearity of the radiochromium release assay for cell-mediated cytotoxicity
| Autor: | John A. Thoma, Gregory J. Thoma, William A. V. Clark |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Male
Lysis Time Factors Somatic cell T-Lymphocytes Immunology chemistry.chemical_element Biology Chromium Mice medicine Neoplasm Bioassay Animals Cell-mediated cytotoxicity Edetic Acid Immunity Cellular Mice Inbred BALB C Isotopes of chromium medicine.disease Cytotoxicity Tests Immunologic Chromium Radioisotopes Mice Inbred C57BL chemistry Toxicity Biophysics Female |
| Zdroj: | Cellular immunology. 40(2) |
| ISSN: | 0008-8749 |
| Popis: | The release of radiochromium from EL-4 cells lysed by anti EL-4 peritoneal lymphocytes is a first order decay process with respect to time. The rate of 51Cr release from damaged cells was independent of the effector: target ratio but was slower from pelleted cells than cells in suspension. We conclude that under normal assay conditions the fraction of chromium released is nearly proportional to the number of cells damaged but find that the proportionality constant is influenced by assay conditions. There is a lag time of 23 ± 5 min before 51Cr is rapidly released from the cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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