Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication
| Autor: | Mikoto Yoshida, Eiji Abe, Mikio Suzuki, Ei Ichi Takahashi, Hidenori Terasaki, Kazuhiko Kuwahara, Yoshinobu Matsuo, Nobuo Sakaguchi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
DNA Replication
DNA Complementary Chromosomes Human Pair 21 Molecular Sequence Data Gene Expression Cell Cycle Proteins Biology Acetyltransferases Transcription (biology) Sequence Homology Nucleic Acid Gene expression Tumor Cells Cultured Genetics Humans Tissue Distribution Amino Acid Sequence RNA Messenger Northern blot Cloning Molecular Gene In Situ Hybridization In Situ Hybridization Fluorescence B-Lymphocytes Messenger RNA Base Sequence Alternative splicing Intracellular Signaling Peptides and Proteins DNA replication Chromosome Mapping Minichromosome Maintenance Complex Component 3 Nuclear Proteins RNA Sequence Analysis DNA General Medicine Blotting Northern Germinal Center Phosphoproteins Immunohistochemistry Molecular biology DNA-Binding Proteins Genes Female Sequence Alignment |
| Zdroj: | Gene. 255:219-227 |
| ISSN: | 0378-1119 |
| Popis: | A 210kDa protein named GANP is upregulated in germinal center (GC)-B cells in the spleen of antigen-immunized mouse. We studied a human ganp gene (hganp) encoding a putative polypeptide of 1980 amino acids. The carboxyl-terminal 721-amino-acid sequence of hGANP is identical to Map80, that is presumably generated by alternative splicing of hganp/Map80 gene. The genomic segment carrying hganp and Map80 genes was isolated, and the chromosomal location was determined on 21q22.3. Northern blot analysis with RNAs from various organs demonstrated a single band of 7kb hganp mRNA, which suggests a preferential transcription of hganp gene from the hganp/Map80 locus. The hGANP expression was upregulated in GCs of the tonsil, as demonstrated by in-situ RNA hybridization and immunohistochemical analyses. The hGANP, with the domain (Map-box) capable of binding to MCM3 in B cells, might be involved in regulation of cell-cycle progression and DNA replication of GC-B cells. |
| Databáze: | OpenAIRE |
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