Influence of SKF38393 on changes of gene profile in rat prefrontal cortex during chronic paradoxical sleep deprivation
| Autor: | Jiangbo Zhu, Yue Tan, Si Chen, Xiaosa Wen, Xinmin Chen, Wenling Ma, Fei Rong |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male HOMER1 Prefrontal Cortex Rats Sprague-Dawley 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Dopamine receptor D1 Homer Scaffolding Proteins Dopamine medicine Animals Circadian rhythm Prefrontal cortex Oligonucleotide Array Sequence Analysis Mitogen-Activated Protein Kinase Kinases Principal Component Analysis Gene Expression Profiling Receptors Dopamine D1 Rats Sleep deprivation 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Dopamine Agonists Sleep Deprivation Glutamatergic synapse Neuron 2 3 4 5-Tetrahydro-7 8-dihydroxy-1-phenyl-1H-3-benzazepine medicine.symptom Psychology Neuroscience 030217 neurology & neurosurgery medicine.drug Signal Transduction |
| Zdroj: | Behavioural brain research. 304 |
| ISSN: | 1872-7549 |
| Popis: | Chronic paradoxical sleep deprivation (CSD) can induce dramatic physiological and neurofunctional changes in rats, including decreased body weight, reduced learning and memory, and declined locomotor function. SKF38393, a dopamine D1 receptor agonist, can reverse the above damages. However, the mechanism of CSD syndrome and reversal role of SKF38393 remains largely unexplained. To preliminarily elucidate the mechanism of the neural dysfunction caused by CSD, in the present study we use gene chips to examine the expression profile of more than 28,000 transcripts in the prefrontal cortex (PFC). Rats were sleep deprived by modified multi-platform method for 3 weeks. Totally 59 transcripts showed differential expressions in CSD group in contrast to controls; they included transcripts coding for caffeine metabolism, circadian rhythm, drug metabolism and some amino acid metabolism pathway. Among the 59 transcripts, 39 increased their expression and 20 decreased. Two transcripts can be specifically reversed with SKF38393, one of them is Homer1, which is related to 20 functional classifications and coding for Glutamatergic synapse pathway. Our findings in the present study indicate that long-term sleep deprivation may trigger the changes of some certain functions and pathways in the PFC, and lead to the dysfunction of this advanced neuron, and the activation of D1 receptor by SKF38393 might ameliorate these changes via modulation of some transcripts such as Homer1, which is involved in the Ca(2+) pathway and MAPK pathway related to Glutamatergic synapse pathway. |
| Databáze: | OpenAIRE |
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