Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
| Autor: | Thomas S. Lisse, Elizabeth A. Brochu, Sandra Rieger |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Tail
Fin Mesenchyme General Chemical Engineering Biology General Biochemistry Genetics and Molecular Biology Fin regeneration 03 medical and health sciences Heated incubation chamber 0302 clinical medicine Stereo microscope Zebrafish larvae medicine Animals Regeneration Issue 95 Zebrafish 030304 developmental biology Microscopy 0303 health sciences General Immunology and Microbiology Post-imaging analysis Time-lapse imaging General Neuroscience fungi Anatomy Tissue repair biology.organism_classification medicine.anatomical_structure Stereomicroscope Developmental biology 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| DOI: | 10.3791/52654-v |
| Popis: | The zebrafish larval tail fin is ideal for studying tissue regeneration due to the simple architecture of the larval fin-fold, which comprises of two layers of skin that enclose undifferentiated mesenchyme, and because the larval tail fin regenerates rapidly within 2-3 days. Using this system, we demonstrate a method for capturing the repair dynamics of the amputated tail fin with time-lapse video brightfield stereomicroscopy. We demonstrate that fin amputation triggers a contraction of the amputation wound and extrusion of cells around the wound margin, leading to their subsequent clearance. Fin regeneration proceeds from proximal to distal direction after a short delay. In addition, developmental growth of the larva can be observed during all stages. The presented method provides an opportunity for observing and analyzing whole tissue-scale behaviors such as fin development and growth in a simple microscope setting, which is easily adaptable to any stereomicroscope with time-lapse capabilities. |
| Databáze: | OpenAIRE |
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