Slicer Endonuclease Argonaute 2 Is a Negative Regulator of Hematopoietic Stem Cell Quiescence
Autor: | Chozhavendan Rathinam, Masahiro Nakagawa, Keyur Thummar, Kenneth Lu |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Cellular differentiation Biology Transcriptome 03 medical and health sciences Bone Marrow medicine Animals Cell Lineage Gene Regulatory Networks Progenitor cell Transcription factor Mice Knockout Genetics Body Weight Cell Cycle Hematopoietic stem cell Cell Differentiation hemic and immune systems Cell Biology Cell cycle Hematopoietic Stem Cells Blood Cell Count Cell biology Haematopoiesis 030104 developmental biology medicine.anatomical_structure Argonaute Proteins Mutation Molecular Medicine Stem cell Transcription Factors Developmental Biology |
Zdroj: | Stem Cells. 34:1343-1353 |
ISSN: | 1549-4918 1066-5099 |
DOI: | 10.1002/stem.2302 |
Popis: | Hematopoietic stem cells (HSCs) are capable of both self-renewing throughout the lifetime of an organism and differentiating into all lineages of the blood system. A proper balance between quiescence and proliferation is critical for the self-renewal and functions of HSCs. The choice of HSCs to remain quiescent or to enter proliferation has been tightly regulated by a variety of cell intrinsic and extrinsic pathways. Identifying molecular players that control HSC quiescence and proliferation may lead to new treatment strategies and therapeutic interventions for hematologic disorders. To identify the functions of the slicer endonuclease Argonaute (Ago) 2 in the physiology of HSCs, we generated Ago2Hem-KO mice, that are deficient for Ago2 in HSCs and in their progeny. Analysis of Ago2Hem-KO mice indicated that a loss of Ago2 results in reduced HSC pool size and altered frequencies of hematopoietic progenitors. Ago2 deficient HSCs exhibit defective multilineage differentiation capacities and diminished repopulation abilities, in a cell intrinsic manner. Interestingly, Ago2 mutant HSCs remain largely quiescent and show reduced entry into cell cycle. Genome-wide transcriptome studies and gene set enrichment analysis revealed that Ago2 deficient HSCs downregulate the “HSC signature” and upregulate the “lineage signature.” Moreover, our analysis on transcription factors (TFs) identified that a loss of Ago2 is sufficient to alter the “molecular signature” and “TF networks” that control the quiescent and proliferative states of HSCs. In essence, our study identified Ago2 as a key determinant of quiescence exit in HSCs. |
Databáze: | OpenAIRE |
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