In Vivo Tissue Response of Endodontic Bio-ceramic Materials

Autor: Liliana Raquel Missana, Ana García Rusco, Kiyofumi Takabatake, Jorge Olmos Fassi, Hitoshi Nagatsuka, Teresita Romano, Keisuke Nakano, Silvia Noemi Kozuszko, María Victoria Jammal
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: In order to evaluate the biocompatibility and mineral repair capacity, mineral trioxide aggregate (MTA ProRoot-Dentsply, Tulsa, Tulsa, Oklahoma-USA), Portland cement (PCn) normal setting (CP; Loma Negra-Argentina) and fast setting (PCf) (CP; Klaukol- Argentina) and Calcium hydroxide-based paste (Calen SS White Dental Articles Ltda.- Brazil) filled silicone tube were implanted into the dorsal subcutaneous connective tissues of 25 Wistar rats. Animals were euthanized at 24, 72 hours, 7, 15 and 30 days. Implants with surrounding tissues were fixed with 10% buffer formaldehyde and processed for histological routine techniques. Slides (5 µ serial cuts) were stained with H&E and Von Kossa stains for morphological, qualitative and quantitative analysis by light microscopy. HOCa2 (Calen) showed severe and moderate inflammatory response and granulomatous reaction with psammoma body-like formation. PCn and MTA have similar behavior, with mild inflammatory reaction from 8 % and 4%, respectively. Even though, PCn and MTA demonstrated analogous biological reaction, MTA developing thick artificial mineral precipitation (p=0,007). All sealers demonstrated a similar inflammatory response at all time periods studied (p=0.678). Fil: Romano, Teresita. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina Fil: Jammal, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Nakano, K.. Okayama Univ, Med.dent.and Pharm Sci. School, Oral Path; Japón Fil: Garcia Rusco, Ana. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina Fil: Olmos Fassi, Jorge. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina Fil: Kozuszko, Silvia. Universidad Nacional de Tucuman. Facultad de Odontologia. Cat.de Anatomia Patologica/laboratorio de Patologia Molecular; Argentina Fil: Takabatake, K.. Okayama Univ, Med.dent.and Pharm Sci. School, Oral Path; Japón Fil: Nagatsuka, H.. Okayama Univ, Med.dent.and Pharm Sci. School, Oral Path; Japón Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Databáze: OpenAIRE