Development of an Inhibition Enzyme-Linked Immunosorbent Assay (ELISA) Prototype for Detecting Cytotoxic Three-Finger Toxins (3FTxs) in African Spitting Cobra Venoms
| Autor: | Ernest Z. Manson, Kyama C. Mutinda, Joseph K. Gikunju, Aleksandra Bocian, Konrad K. Hus, Vladimír Petrílla, Jaroslav Legáth, James H. Kimotho |
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| Rok vydání: | 2021 |
| Předmět: |
Elapid Venoms
Analysis of Variance Mice Inbred BALB C Pharmaceutical Science Organic chemistry Enzyme-Linked Immunosorbent Assay complex mixtures Analytical Chemistry inhibition ELISA cytotoxic three-finger toxins snakebite envenoming diagnostics Mice QD241-441 Chemistry (miscellaneous) Drug Discovery Molecular Medicine Animals Female Elapidae Physical and Theoretical Chemistry Three Finger Toxins |
| Zdroj: | Molecules; Volume 27; Issue 3; Pages: 888 Molecules, Vol 27, Iss 888, p 888 (2022) |
| ISSN: | 1420-3049 |
| Popis: | The administration of toxin-specific therapy in snake envenoming is predicated on improved diagnostic techniques capable of detecting specific venom toxins. Various serological tests have been used in detecting snakebite envenoming. Comparatively, enzyme-linked immunosorbent assay (ELISA) has been shown to offer a wider practical application. We report an inhibition ELISA for detecting three-finger toxin (3FTx) proteins in venoms of African spitting cobras. The optimized assay detected 3FTxs in N. ashei (including other Naja sp.) venoms, spiked samples, and venom-challenged mice samples. In venoms of Naja sp., the assay showed inhibition, implying the detection of 3FTxs, but showed little or no inhibition in non-Naja sp. In mice-spiked samples, one-way ANOVA results showed that the observed inhibition was not statistically significant between spiked samples and negative control (p-value = 0.164). Similarly, the observed differences in inhibition between venom-challenged and negative control samples were not statistically significant (p-value = 0.9109). At an LOD of 0.01 µg/mL, the assay was able to confirm the presence of 3FTxs in the samples. Our results show a proof of concept for the use of an inhibition ELISA model as a tool for detecting 3FTxs in the venoms of African spitting cobra snakes. |
| Databáze: | OpenAIRE |
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