Enlarged colitogenic T cell population paradoxically supports colitis prevention through the B-lymphocyte-dependent peripheral generation of CD4(+)Foxp3(+) Treg cells
| Autor: | Maria Bellio, Luciana Souza de Paiva, Alberto Nobrega, Rita Fucs, Rafael Ferreira da Silva, Alessandra Granato, Sylvia M. N. Campos, Fábio B. Canto |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Naive T cell Lymphocyte T cell chemical and pharmacologic phenomena Biology T-Lymphocytes Regulatory Article 03 medical and health sciences Interleukin 21 Mice 0302 clinical medicine medicine Cytotoxic T cell Animals Mesentery IL-2 receptor Antigen-presenting cell Mice Knockout B-Lymphocytes Multidisciplinary Models Immunological hemic and immune systems Natural killer T cell Colitis 030104 developmental biology medicine.anatomical_structure Immunology Lymph Nodes 030215 immunology |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Intestinal inflammation can be induced by the reconstitution of T/B cell-deficient mice with low numbers of CD4+ T lymphocytes depleted of CD25+Foxp3+ regulatory T cells (Treg). Using RAG-knockout mice as recipients of either splenocytes exclusively depleted of CD25+ cells or FACS-purified CD4+CD25−Foxp3− T cells, we found that the augmentation of potentially colitogenic naïve T cell numbers in the inoculum was unexpectedly beneficial for the suppression of colon disease and maintenance of immune homeostasis. Protection against T cell-mediated colitis correlated with a significant increment in the frequency of peripherally-induced CD4+CD25+Foxp3+ T (pTreg) cells, especially in the mesenteric lymph nodes, an effect that required the presence of B cells and CD4+CD25−Foxp3+ cells in physiological proportions. Our findings support a model whereby the interplay between B lymphocytes and a diversified naïve T cell repertoire is critical for the generation of CD4+CD25+Foxp3+ pTreg cells and colitis suppression. |
| Databáze: | OpenAIRE |
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