Structural characters and protecting β-cells of a polysaccharide from flowers of Inula japonica
| Autor: | Diao Yulin, Chunzhi Zhao, Changzhen Wang, Hao Ma, Junjie Shan, Zhao Xiunan, Guohui Sun, Wensheng Qu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Arabinose Rhamnose Mannose 030209 endocrinology & metabolism Flowers Polysaccharide medicine.disease_cause Biochemistry Cell Line Mice Structure-Activity Relationship 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Polysaccharides Superoxides Structural Biology Insulin-Secreting Cells Alloxan medicine Animals Molecular Biology chemistry.chemical_classification Inula biology Hydroxyl Radical General Medicine biology.organism_classification 030104 developmental biology chemistry Cytoprotection Galactose Oxidative stress |
| Zdroj: | International Journal of Biological Macromolecules. 101:16-23 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2017.03.044 |
| Popis: | Our previous studies found that the crude polysaccharides (IJP) from flowers of Inula japonica exhibited significantly anti-diabetic activity in alloxan or MLD-STZ induced diabetic mice. In this study, we will trace an active polysaccharide from IJP and investigate its physico-chemical property and its protective mechanism on islet cell damage. The result showed that an active polysaccharide (IJP-B-1) was isolated from IJP, its molecular mass was 3.7×104Da. IJP-B-1 was composed of rhamnose, arabinose, xylose, mannose, glucose, galactose and galactocuronic acid. Its major backbone structure was (1→3, 6)-linked-galactose and other branched residues. IJP-B-1 could protect pancreatic cells against STZ impairment at 50μg/mL and scavenge OH and O2 radicals to decrease reactive oxygen generation in islet-cells in vitro. These results suggested that IJP-B-1 might be useful for protecting β-cells and against oxidative stress as an anti-diabetic candidate drug in future. |
| Databáze: | OpenAIRE |
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