Enhanced biocompatibility and improved osteogenesis of coralline hydroxyapatite modified by bone morphogenetic protein 2 incorporated into a biomimetic coating
| Autor: | Xingnan Lin, Yuelian Liu, Qingang Hu, Tie Liu, Ernst B. Hunziker |
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| Přispěvatelé: | Oral Implantology |
| Rok vydání: | 2019 |
| Předmět: |
Male
Ceramics Foreign-body giant cell Materials science Biocompatibility Depot education Bone Morphogenetic Protein 2 Bioengineering 02 engineering and technology engineering.material 010402 general chemistry 01 natural sciences Bone morphogenetic protein 2 Biomaterials chemistry.chemical_compound Coated Materials Biocompatible Coating Biomimetic Materials Osteogenesis Materials Testing parasitic diseases Animals SDG 14 - Life Below Water Rats Wistar 610 Medicine & health Octacalcium phosphate 021001 nanoscience & nanotechnology Delivery mode Rats 0104 chemical sciences chemistry Mechanics of Materials Drug delivery engineering Hydroxyapatites 0210 nano-technology Biomedical engineering |
| Zdroj: | Materials Science & Engineering: C-Materials for Biological Applications, 96, 329-336. Elsevier BV Lin, X, Hunziker, E B, Liu, T, Hu, Q & Liu, Y 2019, ' Enhanced biocompatibility and improved osteogenesis of coralline hydroxyapatite modified by bone morphogenetic protein 2 incorporated into a biomimetic coating ', Materials Science & Engineering: C-Materials for Biological Applications, vol. 96, pp. 329-336 . https://doi.org/10.1016/j.msec.2018.11.017 |
| ISSN: | 0928-4931 |
| Popis: | Objectives (1) To determine whether the biocompatibility of coralline hydroxyapatite (CHA) granules could be improved by using an octacalcium phosphate (OCP) coating layer, and/or functionalized with bone morphogenetic protein 2 (BMP-2), and (2) to investigate if BMP-2 incorporated into this coating is able to enhance its osteoinductive efficiency, in comparison to its surface-adsorbed delivery mode. Methods CHA granules (0.25 g per sample) bearing a coating-incorporated depot of BMP-2 (20 μg/sample) together with the controls (CHA bearing an adsorbed depot of BMP-2; CHA granules with an OCP coating without BMP-2; pure CHA granules) were implanted subcutaneously in rats (n = 6 animals per group). Five weeks later, the implants were retrieved for histomorphometric analysis to quantify the volume of newly generated bone, bone marrow, fibrous tissue and foreign body giant cells (FBGCs). The osteoinductive efficiency of BMP-2 and the rates of CHA degradation were also determined. Results The group with an OCP coating-incorporated depot of BMP-2 showed the highest volume and quality or bone, and the highest osteoinductive efficacy. OCP coating was able to reduce inflammatory responses (improve biocompatibility), and also simple adsorption of BMP-2 to CHA achieved this. Conclusions The biocompatibility of CHA granules (reduction of inflammation) was significantly improved by coating with a layer of OCP. Pure surface adsorption of BMP-2 to CHA also reduced inflammation. Incorporation of BMP-2 into the OCP coatings was associated with the highest volume and quality of bone, and the highest biocompatibility degree of the CHA granules. Clinical significance Higher osteoinductivity and improved biocompatibility of CHA can be obtained when a layer of BMP-2 functionalized OCP is deposited on the surfaces of CHA granules. |
| Databáze: | OpenAIRE |
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