Molecular cloning, sequencing, and characterizing the bovine receptor for gonadotropin releasing hormone (GnRH)
| Autor: | J.D. Neill, C.H. Rahe, S.S. Kakar |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Pituitary gland Transcription Genetic Molecular Sequence Data Gonadotropin-releasing hormone Biology Gonadotropic cell Transfection Cell Line Gonadotropin-Releasing Hormone Open Reading Frames Endocrinology Food Animals Internal medicine Complementary DNA Sequence Homology Nucleic Acid Gene expression medicine Animals Northern blot Amino Acid Sequence RNA Messenger Cloning Molecular Receptor DNA Primers Base Sequence Cell Membrane Blotting Northern Molecular biology medicine.anatomical_structure Gene Expression Regulation Hypothalamus Pituitary Gland Animal Science and Zoology Cattle Female hormones hormone substitutes and hormone antagonists Receptors LHRH |
| Zdroj: | Domestic animal endocrinology. 10(4) |
| ISSN: | 0739-7240 |
| Popis: | Receptors for gonadotropin releasing hormone (GnRH), located in the cell membranes of adenohypophysial gonadotropes, mediate the action of GnRH to stimulate the secretion of the gonadotropic hormones (LH and FSH). In the present studies, we have isolated a GnRH receptor cDNA from bovine pituitary, determined its primary structure, and studied the regulation of its gene expression. The cDNA is composed of 1326 nucleotides and encodes a protein containing 328 amino acids. The GnRH receptor of cattle, like that in humans and mice, is a seven transmembrane receptor and has structural characteristics homologous with the family of G protein-coupled receptors. It exhibits 91% identity at the amino acid level with the human and 86% identity with mouse and rat receptors. Northern blot analysis of the RNA from bovine pituitary, probed with 32 P-labeled bovine GnRH receptor cDNA, revealed the presence of four different transcripts (5.0, 3.5, 2.5 and 1.5 kb) in the pituitary of which the 5.0 kb form was most abundant. Using the reverse transcriptase/PCR technique, we detected expression of GnRH receptor mRNA in the pituitary but not in any other extrapituitary tissues such as the hypothalamus, hippocampus, testis, corpus luteum, ovary (containing follicles), myoendometrium, adrenal, kidney, liver and spleen. Higher levels of GnRH receptor mRNA were found in the pituitaries of steers than in cohort bulls, suggesting regulation of GnRH receptor gene expression by testicular steroids. |
| Databáze: | OpenAIRE |
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