Regulation of follicle growth and apoptosis in cultured mouse ovaries by NGF and oxygen
| Autor: | Jaesook Roh, Woojin Pi |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Drug Evaluation Preclinical Ovary Apoptosis Biology Organ culture Cryopreservation Andrology Mice Endocrinology Ovarian Follicle Internal medicine Proliferating Cell Nuclear Antigen Nerve Growth Factor medicine Animals Cells Cultured Cell Proliferation Cell growth Obstetrics and Gynecology In vitro Oxygen medicine.anatomical_structure Nerve growth factor Oocytes Limiting oxygen concentration Female |
| Zdroj: | Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology. 29(6) |
| ISSN: | 1473-0766 |
| Popis: | Cryopreservation of ovarian tissue has been proposed for use in preserving female fertility before anticancer chemo-radiotherapy, because ovarian tissue contains a large pool of non-growing, primordial follicles. The mechanisms that regulate the exit of follicles from the pool are poorly understood. To determine optimal conditions for in vitro ovarian culture, we investigated the effects of nerve growth factor (NGF) and oxygen concentration on follicle growth and apoptosis. Oxygen concentration affected both cell proliferation and apoptosis. Under 20% oxygen, but not 1.5% or 5%, NGF decreased apoptosis in mouse ovaries by down-regulating the pro-apoptotic genes Bax and p53. In conclusion, high oxygen tension during in vitro ovarian culture promotes follicle growth and, in conjunction with NGF, suppresses apoptosis. The efficiency of this method to preserve fertility depends in part on the level of atresia. These results suggest that oxygen and NGF may be used to increase numbers of preantral follicles and mature oocytes in the culture of mammalian ovarian cortical strips. |
| Databáze: | OpenAIRE |
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