Protein/Arabinoxylans Gels: Effect of Mass Ratio on the Rheological, Microstructural and Diffusional Characteristics
| Autor: | Alma Rosa Islas-Rubio, Alma Rosa Toledo-Guillén, Agustín Rascón-Chu, Claudia M. Berlanga-Reyes, Madhav P. Yadav, Jaime Lizardi-Mendoza, Kevin B. Hicks, Elizabeth Carvajal-Millan |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Dietary Fiber
Serum albumin Protein aggregation Polysaccharide Catalysis Article Inorganic Chemistry Ferulic acid lcsh:Chemistry Diffusion chemistry.chemical_compound Rheology Polysaccharides Arabinoxylan Physical and Theoretical Chemistry Bovine serum albumin Molecular Biology lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification Chromatography biology Chemistry Viscosity Organic Chemistry Proteins Serum Albumin Bovine General Medicine Elasticity Computer Science Applications arabinoxylan lcsh:Biology (General) lcsh:QD1-999 Covalent bond biology.protein Xylans phase separation protein controlled release Gels ferulic acid |
| Zdroj: | International Journal of Molecular Sciences Volume 15 Issue 10 Pages 19106-19118 International Journal of Molecular Sciences, Vol 15, Iss 10, Pp 19106-19118 (2014) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms151019106 |
| Popis: | Wheat bran arabinoxylan (WBAX) gels entrapping standard model proteins at different mass ratios were formed. The entrapment of protein affected the gel elasticity and viscosity values, which decreased from 177 to 138 Pa. The presence of protein did not modify the covalent cross-links content of the gel. The distribution of protein through the network was investigated by confocal laser scanning microscopy. In mixed gels, protein aggregates forming clusters were detected at protein/polysaccharide ratios higher than 0.25. These clusters were not homogeneously distributed, suggesting that WBAX and protein are located in two different phases. The apparent diffusion coefficient (Dm) of proteins during release from mixed gels was investigated for mass ratios of 0.06 and 0.12. For insulin, Dm increased significantly from 2.64 × 10−7 to 3.20 × 10−7 cm2/s as the mass ratio augmented from 0.06 to 0.12. No significant difference was found for Dm values of ovalbumin and bovine serum albumin released from the mixed gels. The results indicate that homogeneous protein/WBAX gels can be formed at low mass ratios, allowing the estimation of Dm by using an analytical solution of the second Fick’s law. |
| Databáze: | OpenAIRE |
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