Expression and antibacterial activity of hybrid antimicrobial peptide cecropinA-thanatin in Pichia pastoris
| Autor: | Xiuqing Wang, Zeqiang Liu, Liyuan Hui, Guimao Yang, Tiantian Yang, Mingxing Zhu, Longmei Yu, Xiangjun Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Expression vector biology 030106 microbiology Antimicrobial peptides lcsh:R lcsh:Medicine Peptide Antimicrobial biology.organism_classification Pichia pastoris 03 medical and health sciences Minimum inhibitory concentration 030104 developmental biology chemistry Biochemistry Antibacterial activity Peptide sequence |
| Zdroj: | Frontiers in Laboratory Medicine, Vol 2, Iss 1, Pp 23-29 (2018) |
| ISSN: | 2542-3649 |
| Popis: | The hybrid peptide CA(1–7)-T(4–19) gene was designed according to the N-terminal 1–7 amino acid sequence of the antimicrobial peptide cecropinA (CA) and the N-terminal 4–19 amino acid sequence of thanatin (T), and synthesized by using Pichia pastoris preferred codons. Pichia pastoris hybrid expression vector was constructed, transformed into the prepared expression strain X-33 competent cells, and regulated by the alcohol oxidase (AOX). The biological activity studies after purification have shown that the hybrid antimicrobial peptides were equipped with broad-spectrum antimicrobial activity, and had a good antimicrobial effect on the majority of Gram-positive and Gram-negative bacteria without hemolysis and good stability in vitro as well. Minimum inhibitory concentration (MIC) of the hybrid peptide on common resistant pathogens in clinics was obtained. It provided a new field for researchers to find ideal antimicrobial agents. Therefore, the antimicrobial peptides have great potential for application. Keywords: Hybrid antimicrobial peptide, CecropinA, Thanatin, Pichia expression system, Antibacterial activity |
| Databáze: | OpenAIRE |
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