Secreted Bacillus anthracis proteases target the host fibrinolytic system
| Autor: | Shelley C. Jorgensen, Myung Chul Chung, Fatah Kashanchi, Charles L. Bailey, Jessica H. Tonry, Serguei G. Popov |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Proteases Carboxypeptidase B2 medicine.medical_treatment Proteolysis Immunology Microbiology Receptors Urokinase Plasminogen Activator Anthrax Rodent Diseases Mice Fibrinolysis Plasminogen Activator Inhibitor 1 medicine Plasminogen Activator Inhibitor 2 Immunology and Allergy Animals Humans Protease biology medicine.diagnostic_test INHA Proteolytic enzymes General Medicine biology.organism_classification Urokinase-Type Plasminogen Activator Recombinant Proteins Bacillus anthracis Disease Models Animal Infectious Diseases Mice Inbred DBA Female Plasminogen activator Peptide Hydrolases |
| Zdroj: | FEMS immunology and medical microbiology. 62(2) |
| ISSN: | 1574-695X |
| Popis: | The fibrinolytic system is often the target for pathogenic bacteria, resulting in increased fibrinolysis, bacterial dissemination, and inflammation. The purpose of this study was to explore whether proteases NprB and InhA secreted by Bacillus anthracis could activate the host's fibrinolytic system. NprB efficiently activated human pro-urokinase plasminogen activator (pro-uPA), a key protein in the fibrinolytic cascade. Conversely, InhA had little effect on pro-uPA. Plasminogen activator inhibitors (PAI)-1, 2 and the uPA receptor were also targets for NprB in vitro. InhA efficiently degraded the thrombin-activatable fibrinolysis inhibitor (TAFI) in vitro. Mice infected with B. anthracis showed a significant decrease in blood TAFI levels. In another mouse experiment, animals infected with isogenic inhA deletion mutants restored TAFI levels, while the levels in the parent strain decreased. We propose that NprB and InhA may contribute to the activation of the fibrinolytic system in anthrax infection. |
| Databáze: | OpenAIRE |
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