Physiological studies related to the immobilization of Penicillium chrysogenum and penicillin production
| Autor: | Christopher Bucke, Gunter Saunders, Taj Keshavarz, Paul J. Mussenden |
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| Rok vydání: | 1993 |
| Předmět: |
Time Factors
Isopenicillin N synthase Bioengineering Penicillins Biology Penicillium chrysogenum Carrageenan Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Biosynthesis medicine RNA Fungal Fungi imperfecti Spores Fungal biology.organism_classification Blotting Northern Spore Penicillin Kinetics chemistry Cell culture Fermentation biology.protein Oxidoreductases medicine.drug Biotechnology |
| Zdroj: | Enzyme and microbial technology. 15(1) |
| ISSN: | 0141-0229 |
| Popis: | Using the production of penicillin by Penicillium chrysogenum as a model system, certain physiological aspects of immobilized and free cell cultures were compared. Reducing the immobilized viable spore loading (from 4 × 104 to 2 × 103 spores ml−1 gel) and initial bead diameter (from 3.5-4.0 to 1.5-2.0 mm) gave rise to an increase in the penicillin titer from 0.2 to 1.2 g l)−1. Using these conditions in immobilized cell culture the growth phase was prolonged and the duration of expression of the isopenicillin N synthase gene (pcbC) was significantly extended when compared with free cell culture (150 h as opposed to 100 h). During the period of maximum penicillin production, different penicillin biosynthetic intermediates accumulated in the broth of free and immobilized cell cultures, reflecting a fundamental difference in cell physiology. Although the maximum specific productivity of penicillin production was reduced by immobilization, the average specific productivity increased when compared to free cell fermentation. |
| Databáze: | OpenAIRE |
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