Expression and function of toll-like receptors in peripheral blood mononuclear cells of patients with polymyalgia rheumatica and giant cell arteritis
| Autor: | Ana Fontalba, Ricardo Blanco, Lorena Álvarez Rodríguez, Marcos López-Hoyos, Jaime Calvo Alen, Maitane Aranzamendi Zaldunbide, Jesús Aguero Balbín, Víctor M. Martínez-Taboada, C. Mata, Jose L. Fernandez-Luna, María José Marín |
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| Rok vydání: | 2011 |
| Předmět: |
Male
T-Lymphocytes Giant Cell Arteritis Immunology Inflammation Peripheral blood mononuclear cell Monocytes General Biochemistry Genetics and Molecular Biology Flow cytometry Polymyalgia rheumatica Rheumatology Humans Immunology and Allergy Medicine Acute-Phase Reaction Receptor Glucocorticoids Aged Aged 80 and over B-Lymphocytes medicine.diagnostic_test business.industry Remission Induction Toll-Like Receptors Acute-phase protein virus diseases Middle Aged medicine.disease Connective tissue disease Giant cell arteritis Toll-Like Receptor 7 Polymyalgia Rheumatica Case-Control Studies Acute Disease Leukocytes Mononuclear Cytokines Female Inflammation Mediators medicine.symptom business |
| Zdroj: | Annals of the Rheumatic Diseases. 70:1677-1683 |
| ISSN: | 0003-4967 |
| DOI: | 10.1136/ard.2010.140194 |
| Popis: | Objective To investigate the expression and function of the Toll-like receptor (TLR) family in peripheral blood mononuclear cells (PBMCs) of patients with polymyalgia rheumatica (PMR) and giant cell arteritis (GCA). Methods The authors analysed 70 patients with PMR, 20 with GCA, and 24 healthy controls (HC). TLR expression was assessed by flow cytometry. TLR function was assessed by stimulating PBMCs with specific ligands. Results A significantly increased expression of TLR7 in PBMCs of patients with active disease compared with HC was found. Despite increased expression of TLR7, circulating monocytes from patients showed a significantly lower in vitro response to TLR7 agonists. No amino acid substitutions predicted to be functionally damaging were found in TLR7. A normal response to specific TLR7 agonists in patients in complete remission eliminated a genetic defect. TLR expression and function were also affected to some degree in other diseases characterised by a strong acute phase response. Conclusion These data suggest activation of TLR7 during the active phase of PMR and GCA which resolves with complete disease remission. Whether this finding is the consequence of the marked inflammatory process in these disorders or activation by natural ligands remains to be explored. |
| Databáze: | OpenAIRE |
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