Association of Laboratory Methods, Colonization Density, and Age With Detection of Streptococcus pneumoniae in the Nasopharynx
| Autor: | Robert Weatherholtz, Janene Colelay, Raymond Reid, Laura L. Hammitt, Michael R. Jacobs, Sopio Chochua, Emily Cloessner, Lindsay R. Grant, Jorge E. Vidal, Mathuram Santosham, Keith P. Klugman, Catherine G. Sutcliffe, Katherine L. O'Brien |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Adolescent Epidemiology White Mountain Apache Physiology Biology medicine.disease_cause Young Adult 03 medical and health sciences 0302 clinical medicine Age groups Culture Techniques Nasopharynx Streptococcus pneumoniae medicine Humans Microbial colonization Colonization Prospective Studies 030212 general & internal medicine Child 0303 health sciences Laboratory methods 030306 microbiology Age Factors Infant Original Contribution Middle Aged Cross-Sectional Studies Real-time polymerase chain reaction Child Preschool Healthy individuals Female |
| Zdroj: | Am J Epidemiol |
| ISSN: | 1476-6256 0002-9262 |
| DOI: | 10.1093/aje/kwz191 |
| Popis: | Culture-based methods for detecting Streptococcus pneumoniae in the nasopharynx lack sensitivity. In this study, we aimed to compare the performance of culture and molecular methods in detecting pneumococcus in the nasopharynx of healthy individuals and to evaluate the associations of age and colonization density with detection. Between 2010 and 2012, nasopharyngeal specimens were collected from healthy individuals living on Navajo Nation and White Mountain Apache Tribal lands in the United States. Pneumococci were detected by means of broth-enrichment culture and autolysin-encoding gene (lytA) quantitative polymerase chain reaction (qPCR). Among 982 persons evaluated (median age, 18.7 years; 47% male), 35% were culture-positive and an additional 27% were qPCR-positive. Agreement between culture and qPCR was 70.9% but was higher among children (age |
| Databáze: | OpenAIRE |
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