Apoptotic, inflammatory, and fibrogenic effects of two different types of multi-walled carbon nanotubes in mouse lung

Autor: C. Albrecht, Thomas A. J. Kuhlbusch, D. van Berlo, Aalt Bast, Maja Hullmann, Agnes W. Boots, R. Schins, Verena Wilhelmi
Přispěvatelé: GezondheidsRisico Analyse en Toxicologie, Farmacologie en Toxicologie, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, RS: NUTRIM - R4 - Gene-environment interaction, RS: CARIM - R3 - Vascular biology
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Pathology
Macrophage
Health
Toxicology and Mutagenesis
Multi-walled carbon nanotubes
Apoptosis
Toxicology
PULMONARY
Fibrosis
PLEURAL INFLAMMATION
Lung
Cell Line
Transformed
GENE-EXPRESSION
Chemistry
MESOTHELIAL CELLS
RAW 264.7 MACROPHAGES
CROCIDOLITE ASBESTOS FIBERS
General Medicine
Specific Pathogen-Free Organisms
medicine.anatomical_structure
Female
medicine.symptom
HEME OXYGENASE-1
medicine.medical_specialty
NONFIBROUS PARTICLES
Chemie
Inflammation
Caspase 3
Respiratory Mucosa
Administration
Inhalation
medicine
Animals
Particle Size
Nanotubes
Carbon
Macrophages
Monocyte
Hydrogen Peroxide
Pneumonia
IN-VITRO
Macrophage Activation
medicine.disease
Molecular biology
Mice
Inbred C57BL
Cell culture
INTRATRACHEAL INSTILLATION
Microscopy
Electron
Scanning
Particulate Matter
Reactive Oxygen Species
Biomarkers
Zdroj: Archives of Toxicology, 88(9), 1725-1737. Springer
ISSN: 0340-5761
Popis: There is increasing concern about the toxicity of inhaled multi-walled carbon nanotubes (MWCNTs). Pulmonary macrophages represent the primary cell type involved in the clearance of inhaled particulate materials, and induction of apoptosis in these cells has been considered to contribute to the development of lung fibrosis. We have investigated the apoptotic, inflammogenic, and fibrogenic potential of two types of MWCNTs, characterised by a contrasting average tube length and entanglement/agglomeration. Both nanotube types triggered H2O2 formation by RAW 264.7 macrophages, but in vitro toxicity was exclusively seen with the longer MWCNT. Both types of nanotubes caused granuloma in the mouse lungs. However, the long MWCNT induced a more pronounced pro-fibrotic (mRNA expression of matrix metalloproteinase-8 and tissue inhibitor of metalloproteinase-1) and inflammatory (serum level of monocyte chemotactic protein-1) response. Masson trichrome staining also revealed epithelial cell hyperplasia for this type of MWCNT. Enhanced apoptosis was detected by cleaved caspase 3 immunohistochemistry in lungs of mice treated with the long and rigid MWCNT and, to a lesser extent, with the shorter, highly agglomerated MWCNT. However, staining was merely localised to granulomatous foci, and neither of the MWCNTs induced apoptosis in vitro, evaluated by caspase 3/7 activity in RAW 264.7 cells. In addition, our study reveals that the inflammatory and pro-fibrotic effects of MWCNTs in the mouse lung can vary considerably depending on their composition. The in vitro analysis of macrophage apoptosis appears to be a poor predictor of their pulmonary hazard.
Databáze: OpenAIRE
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