Steroid Receptor Signalling in the Brain - Lessons Learned from Molecular Imaging
| Autor: | Ken-ichi Matsuda, Hirotaka Sakamoto, Mayumi Nishi, Kazuyo Fujikawa, Keiko Takanami, Hiroko Mori, Y. Hirahara-Wada, Miwako Masugi-Tokita, Natsuko Kaku, Mitsuhiro Kawata |
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| Rok vydání: | 2008 |
| Předmět: |
Receptors
Steroid medicine.medical_specialty Recombinant Fusion Proteins Endocrinology Diabetes and Metabolism medicine.medical_treatment Green Fluorescent Proteins Biology Green fluorescent protein Cellular and Molecular Neuroscience Endocrinology Mineralocorticoid receptor Glucocorticoid receptor Internal medicine polycyclic compounds medicine Animals Receptor Endocrine and Autonomic Systems Cell Membrane Brain Fluorescence recovery after photobleaching Ligand (biochemistry) Androgen receptor Steroid hormone Steroids hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | Journal of Neuroendocrinology. 20:673-676 |
| ISSN: | 1365-2826 0953-8194 |
| Popis: | Studies with green fluorescent protein (GFP) have revealed the subcellular distribution of many steroid hormone receptors to be much more dynamic than previously thought. Fluorescence resonance energy transfer (FRET) and fluorescence recovery after photobleaching (FRAP) are powerful techniques with which to examine protein-protein interaction and the mobility of tagged proteins, respectively. FRET analysis revealed that steroid treatment (with corticosterone or testosterone) induces direct interaction of the glucocorticoid receptor (GR) and importin alpha in the cytoplasm and that, shortly after nuclear entry, the GR detaches from importin alpha. The mineralocorticoid receptor (MR) and androgen receptor (AR) show the same trafficking. Upon oestradiol treatment, ERalpha and ERbeta in the same cell are relocalised to form a discrete pattern and are localised in the same discrete cluster (subnuclear foci). FRAP analysis showed that nuclear ERalpha and ERbeta are most dynamic and mobile in the absence of the ligand, and that mobility decreases slightly after ligand treatment. Genomic as well as non-genomic actions of steroid hormones influence the cellular function of target tissues spacio-temporally. |
| Databáze: | OpenAIRE |
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