A high-protein formula increases colonic peptide transporter 1 activity during neonatal life in low-birth-weight piglets and disturbs barrier function later in life
| Autor: | Isabelle Le Huërou-Luron, Cécile Perrier, Véronique Romé, Agnès Jamin, Gaëlle Boudry, Gérard Savary |
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| Přispěvatelé: | Nutrition, Métabolismes et Cancer (NuMeCan), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), PROTNEONAT-ANR-05-PNRA-09, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-05-PNRA-0009,PROT NEONAT,Nutrition néonatale hyperprotéique : effets sur la maturation intestinale, rénale et hypothalamique chez le nouveau-né, et conséquences à long terme(2005), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, Programme National de Recherches en Alimentation et Nutrition Humaine - Nutrition néonatale hyperprotéique : effets sur la maturation intestinale, rénale et hypothalamique chez le nouveau-né, et conséquences à long terme - - PROT NEONAT2005 - ANR-05-PNRA-0009 - PNRA - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
poids faible de naissance [SDV]Life Sciences [q-bio] Sus scrofa Medicine (miscellaneous) période neonatale Intestinal absorption Jejunum Birth Weight Barrier function ComputingMilieux_MISCELLANEOUS 2. Zero hunger Nutrition and Dietetics Symporters biology Chemistry chambre de ussing peptide [SDV] Life Sciences [q-bio] Intestines medicine.anatomical_structure nutrition Female Dietary Proteins medicine.symptom porcelet medicine.medical_specialty Colon Ileum Inflammation formulation Peptide Transporter 1 Internal medicine Heat shock protein medicine Animals RNA Messenger western blot Peptide transporter 1 Membrane Transport Proteins Infant Low Birth Weight Diet Endocrinology Animals Newborn Intestinal Absorption Symporter Immunology biology.protein absorption |
| Zdroj: | British Journal of Nutrition British Journal of Nutrition, Cambridge University Press (CUP), 2014, 112 (7), pp.1073-1080. ⟨10.1017/S0007114514001901⟩ British Journal of Nutrition 7 (112), 1073-1080. (2014) British Journal of Nutrition, 2014, 112 (7), pp.1073-1080. ⟨10.1017/S0007114514001901⟩ |
| ISSN: | 0007-1145 1475-2662 |
| Popis: | Dietary peptides are absorbed along the intestine through peptide transporter 1 (PepT-1) which is highly responsive to dietary protein level. PepT-1 is also involved in gut homeostasis, both initiating and resolving inflammation. Low-birth-weight (LBW) neonates are routinely fed a high-protein (HP) formula to enhance growth. However, the influence of this nutritional practice on PepT-1 activity is unknown. Intestinal PepT-1 activity was compared in normal-birth-weight (NBW) and LBW piglets. The effect of HP v. normal-protein (NP) formula feeding on PepT-1 activity and gut homeostasis in LBW piglets was evaluated, during the neonatal period and in adulthood. Flux of cephalexin (CFX) across the tissue mounted in Ussing chambers was used as an indicator of PepT-1 activity. CFX flux was greater in the ileum, but not jejunum or colon, of LBW than NBW piglets during the neonatal period. When LBW piglets were formula-fed, the HP formula increased colonic CFX during the 1st week of life. Later in life, intestinal CFX fluxes and barrier function were similar whether LBW pigs had been fed NP or HP formula. However, colonic permeability of HP- but not NP-fed pigs increased when luminal pH was brought to 6·0. The formyl peptide N-formyl methionyl-leucyl-phenylalanine conferred colonic barrier protection in HP-fed piglets. Heat shock protein 27 levels in the colonic mucosa of HP-fed LBW pigs correlated with the magnitude of response to the acidic challenge. In conclusion, feeding a HP formula enhanced colonic PepT-1 activity in LBW pig neonates and increased sensitivity of the colon to luminal stress in adulthood. |
| Databáze: | OpenAIRE |
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