Targeted inactivation of integrin-linked kinase in hair follicle stem cells reveals an important modulatory role in skin repair after injury
| Autor: | Sudhir J.A. D'Souza, Lina Dagnino, Kerry-Ann Nakrieko, Alena Rudkouskaya, Timothy S. Irvine |
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| Rok vydání: | 2011 |
| Předmět: |
Keratinocytes
Cell Physiology medicine.medical_specialty Mice Transgenic Protein Serine-Threonine Kinases Biology Mice 03 medical and health sciences 0302 clinical medicine Internal medicine Keratin otorhinolaryngologic diseases medicine Animals Integrin-linked kinase Gene Silencing Molecular Biology 030304 developmental biology Skin repair chemistry.chemical_classification Wound Healing 0303 health sciences Epidermis (botany) integumentary system Keratin-15 Stem Cells Regeneration (biology) Articles Cell Biology Hair follicle Cell biology Mifepristone Endocrinology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis embryonic structures biology.protein Female sense organs Epidermis Stem cell Wound healing Hair Follicle |
| Zdroj: | Paediatrics Publications Molecular Biology of the Cell |
| Popis: | Inactivation of integrin-linked kinase in the stem cells of the hair follicle bulge results in impaired skin regeneration after injury but does not affect hair follicle entry into anagen. Integrin-linked kinase (ILK) is key for normal epidermal morphogenesis, but little is known about its role in hair follicle stem cells and epidermal regeneration. Hair follicle stem cells are important contributors to newly formed epidermis following injury. We inactivated the Ilk gene in the keratin 15–expressing stem cell population of the mouse hair follicle bulge. Loss of ILK expression in these cells resulted in impaired cutaneous wound healing, with substantially decreased wound closure rates. ILK-deficient stem cells produced very few descendants that moved toward the epidermal surface and into the advancing epithelium that covers the wound. Furthermore, those few mutant cells that homed in the regenerated epidermis exhibited a reduced residence time. Paradoxically, ILK-deficient bulge stem cells responded to anagen growth signals and contributed to newly regenerated hair follicles during this phase of hair follicle growth. Thus ILK plays an important modulatory role in the normal contribution of hair follicle stem cell progeny to the regenerating epidermis following injury. |
| Databáze: | OpenAIRE |
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