Lanosterol Synthase in Dicotyledonous Plants
| Autor: | Toshiya Muranaka, Ting Xiang, Kazuki Saito, Hikaru Seki, Yutaka Ebizuka, Masashi Suzuki, Tetsuo Kushiro, Kiyoshi Ohyama, Masaaki Shibuya, Hiroaki Hayashi, Yuji Katsube |
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| Rok vydání: | 2006 |
| Předmět: |
Squalene
Saccharomyces cerevisiae Proteins DNA Plant Physiology Molecular Sequence Data Arabidopsis Panax Plant Science Genes Plant Lanosterol Magnoliopsida chemistry.chemical_compound Biosynthesis Gene Expression Regulation Plant polycyclic compounds Amino Acid Sequence Intramolecular Transferases Phylogeny biology Arabidopsis Proteins Phytosterol fungi Nuclear Proteins Phytosterols food and beverages Cell Biology General Medicine biology.organism_classification Cycloartenol synthase Biochemistry chemistry biology.protein Cycloartenol lipids (amino acids peptides and proteins) Heterologous expression Lanosterol synthase |
| Zdroj: | Plant and Cell Physiology. 47:565-571 |
| ISSN: | 1471-9053 0032-0781 |
| Popis: | Sterols are important as structural components of plasma membranes and precursors of steroidal hormones in both animals and plants. Plant sterols show a wide structural variety and significant structural differences from those of animals. To elucidate the origin of structural diversity in plant sterols, their biosynthesis has been extensively studied [Benveniste (2004) Annu. Rev. Plant. Biol. 55: 429, Schaller (2004) Plant Physiol. Biochem. 42: 465]. The differences in the biosynthesis of sterols between plants and animals begin at the step of cyclization of 2,3-oxidosqualene, which is cyclized to lanosterol in animals and to cycloartenol in plants. However, here we show that plants also have the ability to synthesize lanosterol directly from 2,3-oxidosqualene, which may lead to a new pathway to plant sterols. The Arabidopsis gene At3g45130, designated LAS1, encodes a functional lanosterol synthase in plants. A phylogenetic tree showed that LAS1 belongs to the previously uncharacterized branch of oxidosqualene cyclases, which differs from the cycloartenol synthase branch. Panax PNZ on the same branch was also shown to be a lanosterol synthase in a yeast heterologous expression system. The higher diversity of plant sterols may require two biosynthetic routes in steroidal backbone formation. |
| Databáze: | OpenAIRE |
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