A Study to Evaluate the Need for Comminution in the Preparation of Food Materials for Testing of Listeria monocytogenes, Staphylococcus Species, and Generic Escherichia coli
| Autor: | Patty Lewandowski, Diane Pickett, Nicole Mitchell, S Brian Caudle, Cameron Owens, Lyndsey Caulkins, Patricia Hanson |
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| Rok vydání: | 2019 |
| Předmět: |
Analyte
Staphylococcus Food Contamination medicine.disease_cause Analytical Chemistry 03 medical and health sciences 0404 agricultural biotechnology 0302 clinical medicine Listeria monocytogenes medicine Enumeration Escherichia coli Environmental Chemistry Sample preparation Staphylococcus species Pharmacology 04 agricultural and veterinary sciences Pulp and paper industry 040401 food science 030220 oncology & carcinogenesis Food Microbiology Environmental science Aseptic processing Comminution Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC International. 103(6) |
| ISSN: | 1944-7922 |
| Popis: | Background Comminution reduces the sampling error arising from distributional heterogeneity of the target contaminant/target analyte in the material, facilitating the selection of a more representative test portion. A laboratory sampling method incorporating comminution prior to selection of the test portion (Sampling Method B) was compared to current sampling methods that used no comminution step (Sampling Method A). Objective This required the development of an efficient process for comminution of food samples prior to removal of the test portion for the detection and isolation of Listeria monocytogenes and the enumeration of Staphylococcus species and Escherichia coli. Method From December 2016 to December 2017, 2742 tests were conducted on 778 unique food samples. For all food samples, a test portion (TPA) was first removed using Sampling Method A, and then the remainder of the material was comminuted and a second test portion (TPB) was removed using Sampling Method B and tested alongside the first portion. Results Across all food matrices and microbial targets, 17 additional targets were detected using only Sampling Method B, and positive detections of target analytes increased by 77% using Sampling Method B from the test portions taken using Sampling Method A. Conclusion Utilizing a sample preparation method that includes a comminution step resulted in an increased number of pathogen detections. Highlights The introduction of a comminution step in the preparation of food samples for detection of three common microbial contaminants resulted in an increase in the rate of detection of natural contaminates in a variety of ready to eat foods. An efficient aseptic process for commutation that can be adapted to a wide range of laboratory settings was identified. |
| Databáze: | OpenAIRE |
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