Determination of Na+/Cl−, Na+/HCO3− and Na+/K+/2Cl− co-transporter activity in corneal endothelial cell plasma membrane vesicles
| Autor: | Christopher G. Wigham, Stuart Hodson, Jason R Lane |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Corneal endothelium
Bicarbonate transport Sodium-Potassium-Chloride Symporters Sodium Inorganic chemistry Membrane vesicle Biophysics chemistry.chemical_element Sodium transport Biochemistry Cotransporter Chlorides medicine Animals Na+/K+-ATPase Symporters Vesicle Sodium-Bicarbonate Symporters Endothelium Corneal Biological Transport Cell Biology Sodium Chloride Symporters Amiloride Bicarbonates chemistry Symporter Potassium Cattle Carrier Proteins medicine.drug Nuclear chemistry |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1328(2):237-242 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/s0005-2736(97)00101-6 |
| Popis: | Corneal endothelial cell derived plasma membrane vesicles were used to investigate the presence of Na+/Cl−, Na+/HCO3− and Na+/K+/2Cl− co-transporter activity in the plasma membranes of these cells. Na+/H+ exchange was blocked by the presence of 1 mM amiloride in all determinations. The rate of accumulation of Na+ in the presence of chloride or bicarbonate was not significantly different from its accumulation in the presence of acetate, thiocyanate or gluconate. The addition of K+ to Na+ plus Cl− did not stimulate Na+ accumulation into the vesicles. The present work provides no evidence for Na+/K+/2Cl−, Na+/Cl− or Na+/HCO3− co-transport in corneal endothelial cell plasma membrane vesicles. |
| Databáze: | OpenAIRE |
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