Stringent starvation protein A and LuxI/LuxR-type quorum sensing system constitute a mutual positive regulation loop in Pseudoalteromonas
| Autor: | Yifan Zhao, Yaoyang Wang, Wei Zhang, Jianhua Yin, Zeyu Hu, Mengting Zhang, Shuangjia Li, Shijun Wu, Jiadi Zhang, Zhiliang Yu, Qiu Meng, Mengdan Ding |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Biophysics Regulator Acyl-Butyrolactones medicine.disease_cause Biochemistry 03 medical and health sciences 0302 clinical medicine Pseudoalteromonas Bacterial Proteins Sigma factor Transcription (biology) Escherichia coli medicine Nucleoid Molecular Biology biology Escherichia coli Proteins fungi Quorum Sensing food and beverages Cell Biology biochemical phenomena metabolism and nutrition biology.organism_classification Cell biology Repressor Proteins Quorum sensing 030104 developmental biology 030220 oncology & carcinogenesis Trans-Activators bacteria rpoS Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 534:885-890 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2020.10.082 |
| Popis: | Bacteria commonly exhibit social activities through acyl-homoserine lactones (AHLs)-based quorum sensing (QS) systems to form their unique social network. The sigma factor RpoS is an important regulator that controls QS system in different bacteria. However, the upstream of RpoS involving regulation on QS system remains unclear. In Escherichia coli RpoS is regulated by stringent starvation protein A (SspA), which is dependent of histone-like nucleoid structuring protein (H-NS). To date, the connection between SspA and QS system is essentially unknown. Here, we characterized a typical LuxI/LuxR-type QS system in marine bacterium Pseudoalteromonas sp. T1lg65 which can produce four types of AHLs. The luxI encoding AHLs synthase and luxR encoding AHLs-responsive receptor are co-transcribed, providing advantages in rapidly amplifying QS signaling. Notably, SspA positively regulated luxI/luxR transcription by activating RpoS expression, which is mediated by H-NS. Interestingly, LuxR in turn positively regulated SspA expression. Therefore, SspA and QS system constitute a mutual positive regulation loop in T1lg65. In view of the crucial roles of SspA and QS system in environmental adaption, we believe that the improvement of bacterial tolerance to marine environments could be related to rapidly tuning SspA-involved QS programming. |
| Databáze: | OpenAIRE |
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