Calsyntenin-1 Regulates Targeting of Dendritic NMDA Receptors and Dendritic Spine Maturation in CA1 Hippocampal Pyramidal Cells during Postnatal Development
| Autor: | Martin Steuble, Urs Gerber, Peter Sonderegger, Clara Orlando, Alexander Akhmedov, Olivier Raineteau, Vincent Pernet, Jeanne Ster, Tu-My Diep |
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| Přispěvatelé: | University of Zurich, Gerber, Urs |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Dendritic spine
Dendritic Spines 610 Medicine & health Neurotransmission Biology Receptors N-Methyl-D-Aspartate Mice medicine 10019 Department of Biochemistry Calsyntenin Animals Receptor CA1 Region Hippocampal Mice Knockout 10242 Brain Research Institute General Neuroscience Pyramidal Cells Calcium-Binding Proteins 2800 General Neuroscience Excitatory Postsynaptic Potentials Long-term potentiation Articles Dendrites Cell biology medicine.anatomical_structure Dendritic transport nervous system Synapses NMDA receptor 570 Life sciences biology Pyramidal cell |
| Zdroj: | Journal of Neuroscience |
| Popis: | Calsyntenin 1 is a transmembrane cargo docking protein important for kinesin 1 mediated fast transport of membrane bound organelles that exhibits peak expression levels at postnatal day 7. However its neuronal function during postnatal development remains unknown. We generated a knock out mouse to characterize calsyntenin 1 function in juvenile mice. In the absence of calsyntenin 1 synaptic transmission was depressed. To address the mechanism evoked EPSPs were analyzed revealing a greater proportion of synaptic GluN2B subunit containing receptors typical for less mature synapses. This imbalance was due to a disruption in calsyntenin 1 mediated dendritic transport of NMDA receptor subunits. As a consequence of increased expression of GluN2B subunits NMDA receptor dependent LTP was enhanced at Schaffer collateral CA1 pyramidal cell synapses. Interestingly these defects were accompanied by a decrease in dendritic arborization and increased proportions of immature filopodia like dendritic protrusions at the expense of thin type dendritic spines in CA1 pyramidal cells. Thus these results highlight a key role for calsyntenin 1 in the transport of NMDA receptors to synaptic targets which is necessary for the maturation of neuronal circuits during early development. © 2014 the authors. |
| Databáze: | OpenAIRE |
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