Amylolytic activity of IgM and IgG antibodies from patients with multiple sclerosis
| Autor: | Tat'yana G. Kanyshkova, Boris M. Doronin, Valentina N. Buneva, Anna A. Kulminskaya, Andrew N. Saveliev, Nadezhda A. Ershova, Georgy A. Nevinsky, Kirill N. Neustroev, Alexander S. Mogelnitskii, Dina R. Ivanen, Olga O. Favorova |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Multiple Sclerosis Igm antibody Immunology Oligosaccharides Antibodies Catalytic chemistry.chemical_compound Fab Fragments medicine Immunology and Allergy Humans chemistry.chemical_classification MS multiple sclerosis Glycogen biology Chemistry Multiple sclerosis Hydrolysis Middle Aged medicine.disease Molecular biology Orders of magnitude (mass) Enzyme Immunoglobulin M Immunoglobulin G Amylases biology.protein Electrophoresis Polyacrylamide Gel Female Antibody |
| Zdroj: | Immunology letters. 86(3) |
| ISSN: | 0165-2478 |
| Popis: | IgG and IgM antibodies from the sera of patients with multiple sclerosis (MS) were found to possess amylolytic activity hydrolyzing α-(1→4)-glucosyl linkages of maltooligosaccharides, glycogen, and several artificial substrates. Individual IgM fractions isolated from 54 analyzed patients with the clinically definite diagnoses of MS had approximately three orders of magnitude higher specific amylolytic activity than that for healthy donors, whereas IgG from only a few patients had high amylolytic activity. Strict criteria were used to prove that the amylolytic activity of IgMs and IgGs is their intrinsic property and is not due to any enzyme contamination. Fab fragments produced from IgM and IgG fractions of the MS patients displayed the same amylolytic activity. IgMs from various patients demonstrated different modes of action in hydrolyzing maltooligosaccharides. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect