Effects of a high fat or a balanced omega 3/omega 6 diet on cytokines levels and DNA damage in experimental colitis
| Autor: | Carlos Augusto Real Martinez, Alessandra Gambero, Vera Lucia Flor Silveira, Gilclay G. Abreu, Patrícia de Oliveira Carvalho, Marcelo Lima Ribeiro, Roberta Araujo Navarro Xavier, Karina V. Barros |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty food.ingredient Colon DNA damage Endocrinology Diabetes and Metabolism Biology Soybean oil Lipid peroxidation Interferon-gamma chemistry.chemical_compound Fish Oils food Risk Factors Fatty Acids Omega-6 Internal medicine Fatty Acids Omega-3 medicine Animals Rats Wistar Colitis Unsaturated fatty acid chemistry.chemical_classification Nutrition and Dietetics Dextran Sulfate Fish oil medicine.disease Dietary Fats Ulcerative colitis Diet Interleukin-10 Rats Soybean Oil Disease Models Animal Endocrinology chemistry Colitis Ulcerative Interleukin-4 DNA Damage Polyunsaturated fatty acid |
| Zdroj: | Nutrition. 27:221-226 |
| ISSN: | 0899-9007 |
| DOI: | 10.1016/j.nut.2009.11.014 |
| Popis: | Objective High-fat diets have been shown to be a risk factor for ulcerative colitis (UC). Omega-6 polyunsaturated fatty acids are considered to increase lipid peroxidation, while the omega-3 polyunsaturated fatty acid exerts a chemopreventative effect. We evaluated the effect of high-fat diets (20%) enriched with fish or soybean oil on colonic inflammation and DNA damage in dextran sulfate sodium-induced colitis. Methods Male Wistar rats (28–30 days) were fed an American Institute of Nutrition (AIN)-93 diet for 47 days and divided into five groups: control normal fat non-colitic (C) or control colitis (CC), high soybean fat group (HS) colitis, high fish fat group colitis, or high-fat soybean plus fish oil colitis. UC was induced from day 35 until day 41 by 3% dextran sulfate sodium. On day 47, the rats were anesthetized; blood samples collected for corticosterone determination, and the distal colon was excised to quantify interleukin-4 (IL-4), IL-10, and interferon-gamma levels, myeloperoxidase activity, histological analyses, and DNA damage. The disease activity index was recorded daily. Results The disease activity index, histological analysis, myeloperoxidase activity, IL-4, interferon-gamma, and corticosterone levels did not differ among the colitic groups. IL-10 was significantly increased by the high fish fat group diet in relation to HS, but only the high soybean-fish fat diet increased the IL-10/IL-4 ratio (anti-inflammatory/pro-inflammatory) to levels closer to the C group and reduced DNA damage compared to the HS group ( P Conclusion The data show that high-fat diets did not exacerbate UC and suggest that the soybean and fish oil mixture, more than the fish oil alone, could be a complementary therapy to achieve a cytokine balance in UC. |
| Databáze: | OpenAIRE |
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