Crystallization and diffraction analysis of the SARS coronavirus nsp10-nsp16 complex
| Autor: | Claire Debarnot, Bruno Canard, Isabelle Varlet, François Ferron, Laure Gluais, Mickaël Bouvet, Etienne Decroly, Julien Lescar, Nicolas Papageorgiou, Isabelle Imbert |
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| Přispěvatelé: | Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Architecture et fonction des macromolécules biologiques (AFMB), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Division Eau et Environnement (LCPC/EAU), Laboratoire Central des Ponts et Chaussées (LCPC)-PRES Université Nantes Angers Le Mans (UNAM), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Methyltransferase
viruses Molecular Sequence Data Biophysics MESH: SARS Virus RNA-dependent RNA polymerase Biology Viral Nonstructural Proteins Crystallography X-Ray Biochemistry law.invention 03 medical and health sciences Structural Biology law MESH: Methyltransferases Genetics Viral structural protein Humans Cap formation MESH: Cloning Molecular Crystallization Cloning Molecular [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] skin and connective tissue diseases Gene 030304 developmental biology MESH: Crystallization 0303 health sciences Messenger RNA MESH: Humans MESH: Molecular Sequence Data 030302 biochemistry & molecular biology fungi RNA virus Methyltransferases Condensed Matter Physics biology.organism_classification RNA-Dependent RNA Polymerase MESH: Crystallography X-Ray Virology 3. Good health [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] body regions Severe acute respiratory syndrome-related coronavirus Crystallization Communications MESH: Viral Nonstructural Proteins MESH: RNA Replicase |
| Zdroj: | Acta crystallographica. Section F, Structural biology communications Acta crystallographica. Section F, Structural biology communications, John Wiley & Sons Ltd, 2011, 67 (Pt 3), pp.404-8. ⟨10.1107/S1744309111002867⟩ Acta Crystallographica Section F Structural Biology and Crystallization Communications Acta crystallographica Section F : Structural biology communications [2014-...] Acta crystallographica Section F : Structural biology communications [2014-..], 2011, 67 (Pt 3), pp.404-8. ⟨10.1107/S1744309111002867⟩ |
| ISSN: | 2053-230X |
| Popis: | International audience; To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1-16), amongst which two methyltransferases, nsp14 and nsp16, are involved in viral mRNA cap formation. The crystal structure of nsp16 is unknown. Nsp16 is an RNA-cap AdoMet-dependent (nucleoside-2'-O-)-methyltransferase that is only active in the presence of nsp10. In this paper, the expression, purification and crystallization of nsp10 in complex with nsp16 are reported. The crystals diffracted to a resolution of 1.9 Å resolution and crystal structure determination is in progress. |
| Databáze: | OpenAIRE |
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