Single-tear proteomics: A feasible approach to precision medicine
| Autor: | Rita Grandori, Antonino Natalello, Alessandro Duse, Dario Di Silvestre, Silvia Tavazzi, Diletta Ami, Antonella De Palma, Fabio Pezzoli, Erika Ponzini, Pierluigi Mauri, Carlo Santambrogio |
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| Přispěvatelé: | Ponzini, E, Ami, D, Duse, A, Santambrogio, C, De Palma, A, Di Silvestre, D, Mauri, P, Pezzoli, F, Natalello, A, Tavazzi, S, Grandori, R |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Proteomics peripheral body fluids single-tear analysis Proteome QH301-705.5 Quantitative proteomics Withdrawal time Computational biology Catalysis Article lacrimal film Inorganic Chemistry Young Adult liquid biopsies personalized medicine mass-spectrometry-based proteomics Liquid biopsie Medicine Humans Physical and Theoretical Chemistry Precision Medicine Biology (General) Shotgun proteomics Eye Proteins Molecular Biology QD1-999 Spectroscopy Biomedicine Morning business.industry Organic Chemistry General Medicine Precision medicine Healthy Volunteers Computer Science Applications Chemistry Peripheral body fluid Tears Single-tear analysi Female Personalized medicine business Biomarkers Mass-spectrometry-based proteomic |
| Zdroj: | International journal of molecular sciences 22 (2021). doi:10.3390/ijms221910750 info:cnr-pdr/source/autori:Ponzini E.; Ami D.; Duse A.; Santambrogio C.; De Palma A.; Di Silvestre D.; Mauri P.; Pezzoli F.; Natalello A.; Tavazzi S.; Grandori R./titolo:Single-tear proteomics: A feasible approach to precision medicine/doi:10.3390%2Fijms221910750/rivista:International journal of molecular sciences (Print)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:22 International Journal of Molecular Sciences, Vol 22, Iss 10750, p 10750 (2021) International Journal of Molecular Sciences; Volume 22; Issue 19; Pages: 10750 International Journal of Molecular Sciences |
| DOI: | 10.3390/ijms221910750 |
| Popis: | Lacrimal fluid is an attractive source of noninvasive biomarkers, the main limitation being the small sample amounts typically collected. Advanced analytical methods to allow for proteomics profiling from a few microliters are needed to develop innovative biomarkers, with attractive perspectives of applications to precision medicine. This work describes an effective, analytical pipeline for single-tear analysis by ultrahigh-resolution, shotgun proteomics from 23 healthy human volunteers, leading to high-confidence identification of a total of 890 proteins. Highly reproducible quantification was achieved by either peak intensity, peak area, or spectral counting. Hierarchical clustering revealed a stratification of females vs. males that did not emerge from previous studies on pooled samples. Two subjects were monitored weekly over 3 weeks. The samples clustered by withdrawal time of day (morning vs. afternoon) but not by follow-up week, with elevated levels of components of the immune system in the morning samples. This study demonstrates feasibility of single-tear quantitative proteomics, envisaging contributions of this unconventional body fluid to individualized approaches in biomedicine. |
| Databáze: | OpenAIRE |
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