Gene amplification and protein overexpression of EGFR and ERBB2 in sinonasal squamous cell carcinoma
| Autor: | José Luis Llorente, Bartolomé Scola, Blanca Vivanco, Mario Hermsen, Carlos Martín Oviedo, Fernando López, Cristina García-Inclán, César Álvarez Marcos |
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| Rok vydání: | 2011 |
| Předmět: |
Oncology
Male Cancer Research medicine.medical_specialty Receptor ErbB-2 Gene Dosage medicine.disease_cause Internal medicine Gene duplication medicine Humans Epidermal growth factor receptor Copy-number variation EGFR Protein Overexpression Aged Aged 80 and over biology medicine.diagnostic_test business.industry Gene Amplification Cancer Genes erbB-1 Genes erbB-2 Middle Aged medicine.disease Prognosis ErbB Receptors Mutation biology.protein Carcinoma Squamous Cell Immunohistochemistry Female KRAS business Paranasal Sinus Neoplasms Fluorescence in situ hybridization |
| Zdroj: | Cancer. 118(7) |
| ISSN: | 1097-0142 |
| Popis: | BACKGROUND: Sinonasal squamous cell carcinomas (SNSCCs) are rare tumors with no etiologic link to tobacco or alcohol, as opposed to other squamous cell carcinomas of the head and neck. Despite improvements in the field of surgery and radiotherapy, patients with these tumors still face a very unfavorable prognosis, partly because of their localization in a complex anatomic area, which has special relevance for surgery and postoperative treatment. Therefore, there is a need for new therapeutic possibilities for patients with these tumors. METHODS: Gene copy numbers of epidermal growth factor receptor (EGFR) and v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 (ERBB2) were analyzed by fluorescence in situ hybridization and multiplex ligand-dependent probe amplification, and protein expression was evaluated by immunohistochemistry in 54 SNSCC specimens. The results were correlated with clinicopathologic and follow-up data. RESULTS: EGFR gene copy number increases were observed in 20 of 45 tumors (44%), and 21 of 54 tumors (39%) had EGFR protein overexpression. Eight of 38 tumors (21%) had ERBB2 copy number increases, and 4 of 54 tumors (7%) exhibited elevated protein expression levels. Both copy number increases and protein overexpression of EGFR and ERBB2 were mutually exclusive. v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations were absent in 37 tumors that were analyzed. CONCLUSIONS: A substantial proportion of SNSCCs carried alterations in EGFR or ERBB2. Together with the absence of KRAS mutations, these findings indicate that therapies targeting these molecules may be promising additions to the therapeutic options for patients with SNSCC. Cancer 2012;. © 2011 American Cancer Society. |
| Databáze: | OpenAIRE |
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