Polymorphisms in TLR4 Gene Associated With Somatic Cell Score in Water Buffaloes (Bubalus bubalis)
| Autor: | Ana Cláudia de Freitas, Gregório Miguel Ferreira de Camargo, Valentina Roldan-Montes, D.J.A. Santos, Ana Cristina Herrera, Humberto Tonhati, Naudin Hurtado-Lugo, Diercles Francisco Cardoso, Daiane Cristina Becker Scalez, Lucia Galvão de Albuquerque, André Vieira do Nascimento |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), University of Guelph, Universidad Francisco de Paula Santander, University of Maryland, Universidade Federal da Bahia (UFBA) |
| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
Somatic cell SNP Single-nucleotide polymorphism mastitis law.invention milk yield 0403 veterinary science 03 medical and health sciences law medicine stop codon Coding region Udder Gene Polymerase chain reaction Original Research 030304 developmental biology Genetics 0303 health sciences lcsh:Veterinary medicine General Veterinary biology udder health 04 agricultural and veterinary sciences medicine.disease biology.organism_classification Mastitis medicine.anatomical_structure lcsh:SF600-1100 molecular marker analysis Veterinary Science Bubalus |
| Zdroj: | Frontiers in Veterinary Science Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Frontiers in Veterinary Science, Vol 7 (2020) |
| ISSN: | 2297-1769 |
| DOI: | 10.3389/fvets.2020.568249 |
| Popis: | Made available in DSpace on 2021-06-25T11:07:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-05 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Considering the importance of the diseases affecting the productive performance of animals in the dairy industry worldwide, it is necessary to implement tools that help to control and limit the occurrence of such diseases. As the increased somatic cell counts (SCC) are a direct expression of the inflammatory process, they are candidates to become the usual parameter for assessing udder health regarding milk quality and for monitoring mastitis incidences. Toll-Like Receptors are membrane proteins that play a key role in immunity, recognizing pathogens and, subsequently, activating immune responses. The present study was conducted to identify single nucleotide polymorphisms in the TLR4 gene of buffaloes and to analyze its associations with somatic cell counts. DNA samples of 120 Murrah buffaloes were used. The whole coding region of the TLR4 gene was amplified by polymerase chain reaction reactions and sequenced for polymorphism scanning. A total of 13 polymorphisms were identified for the sequenced regions of the TLR4, most of which are in the coding region. The association with the somatic cell score was highly significant (p < 0.001) for all identified polymorphisms of TLR4 gene (g.54621T>A, g.54429G>T, g.54407T>A, g.46616C>A, g.46613T>G, g.46612A>G, g.46611C>A, g.46609T>G, g.46541C>G, g.46526C>A, g.46516T>C, g.46376C>T, g.46372T>C). Therefore, it is suggested that the markers of the TLR4 gene can be used as molecular markers for mastitis resistance in buffaloes, due to their association with somatic cell counts. Department of Animal Science School of Agricultural and Veterinarian Science São Paulo State University (UNESP) Department of Animal Biosciences Centre for Genetic Improvement of Livestock University of Guelph Facultad de Ciências Agrárias Universidad Francisco de Paula Santander Department of Animal and Avian Sciences University of Maryland Escola de Medicina Veterinária e Zootecnia Universidade Federal da Bahia Department of Animal Science School of Agricultural and Veterinarian Science São Paulo State University (UNESP) |
| Databáze: | OpenAIRE |
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