Effects of sodium salicylate and time postpartum on mammary tissue proliferation, gene transcript profile, and DNA methylation
| Autor: | T.H. Swartz, Laman K. Mamedova, Pablo J. Ross, A.J. Carpenter, K.M. Daniels, S.L. Laflin, Barry J. Bradford, C.M. Ylioja |
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| Rok vydání: | 2021 |
| Předmět: |
Sodium Salicylate
Mammary gland Biology Andrology Transcriptome chemistry.chemical_compound Lactation Gene expression Genetics medicine Animals Sodium salicylate Cell Proliferation Postpartum Period DNA Methylation Prolactin Epithelium Milk medicine.anatomical_structure chemistry DNA methylation Cattle Female Animal Science and Zoology Food Science |
| Zdroj: | Journal of Dairy Science. 104:11259-11276 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2020-20109 |
| Popis: | Previous studies have demonstrated nonsteroidal antiinflammatory drug treatment in early lactation had a positive impact on whole-lactation milk production in older cows. The objective of this study was to evaluate proliferative, transcriptional, and epigenetic changes in the mammary gland that could explain increased production responses due to nonsteroidal antiinflammatory drug treatment. Sodium salicylate (SAL; 125 g/d) or water (CON) were administered via oral drench to multiparous Holstein cows (n = 8/treatment) once daily for 3 d beginning approximately 24 h after parturition, and mammary tissue was collected on d 1, 4, and 45 postpartum. Day 1 tissue was collected immediately preceding the initial drench, and d 4 tissue was collected 24 h following the final drench. Blood was collected twice weekly and analyzed for plasma glucose, insulin, β-hydroxybutyrate, free fatty acids, and prolactin. Cows were milked twice daily until d 7 of lactation, and thrice daily for the remainder of the study. Total RNA extracted from tissue was deep-sequenced and analyzed for differential gene expression using DESeq2. We detected no treatment effect on milk yield or plasma metabolites through 45 d of lactation; additionally, no change in mammary epithelial cell proliferation was detected when assessed by Ki67 labeling. Comparison of SAL versus CON revealed that only 16 of 18,286 genes were differentially expressed (false discovery rate |
| Databáze: | OpenAIRE |
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