Accuracy of herpes simplex virus detection in liquid-based (SurePath) Papanicolaou tests: a comparison with polymerase chain reaction
| Autor: | Stefan E. Pambuccian, Frances L. Prekker, H. Evin Gulbahce, Dan M. McKeon, Timothy W. Schacker, Deniz L. Aslan, Peter J. Southern, Kay Savik |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Histology Adolescent viruses Papanicolaou stain HSL and HSV medicine.disease_cause Polymerase Chain Reaction Pathology and Forensic Medicine law.invention law Cytology Medicine Humans Mass Screening Simplexvirus Longitudinal Studies Polymerase chain reaction Aged Aged 80 and over Vaginal Smears business.industry Herpes Simplex General Medicine Papanicolaou Test Middle Aged Virology Herpes simplex virus Real-time polymerase chain reaction DNA Viral Vagina Female Primer (molecular biology) business |
| Zdroj: | Diagnostic cytopathology. 36(2) |
| ISSN: | 8755-1039 |
| Popis: | A review of our institution's Papanicolaou test records over an 11-yr period showed that liquid-based Papanicolaou tests (LBPTs) had a significantly higher frequency of diagnoses of Herpes simplex virus (HSV)-related cellular changes compared to conventional Papanicolaou smears (77/302,841, 0.026% vs. 56/376,173, 0.015%, P = 0.002). To investigate the accuracy of the diagnosis of HSV by LBPT, we performed conventional polymerase chain reaction (PCR) on the residual samples from 258 prospectively collected LBPT and real-time PCR using a different primer set on a subset of 40 LBPT. Conventional PCR was positive in 22 of 22 cases diagnosed of HSV, 1 of 2 cases diagnosed as suspicious for HSV, and none of 234 LBPT without a cytologic HSV diagnosis. Real-time PCR was positive in 8 of 8 cases diagnosed as HSV and none of the 32 controls. We conclude that LBPT allows an increased detection of HSV that is highly accurate. Diagn. Cytopathol. 2008;36:94–103. © 2008 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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