Tissue distribution of amyloid P component as defined by a monoclonal antibody produced by immunization with human glomerular basement membranes
| Autor: | Huda Al-Mutlaq, A. R. Morley, C Watchorn, Helen Robertson, Janice Wheeler |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pathology
medicine.medical_specialty Amyloid Renal glomerulus medicine.drug_class Immunocytochemistry Kidney Glomerulus Enzyme-Linked Immunosorbent Assay Monoclonal antibody Renal amyloidosis Basement Membrane Immunoenzyme Techniques Mice medicine Animals Humans Tissue Distribution Kidney Mice Inbred BALB C biology Immunoperoxidase Chemistry Antibodies Monoclonal Cell Biology Serum Amyloid P-Component medicine.anatomical_structure biology.protein Electrophoresis Polyacrylamide Gel Anatomy Antibody |
| Zdroj: | The Histochemical journal. 25(3) |
| ISSN: | 0018-2214 |
| Popis: | A monoclonal antibody reactive against amyloid P component (NCL-AMP) has been developed following immunization of mice with partially-purified human glomerular basement membranes (GBM) and standard hybridization and cloning techniques. The antibody reactivity was evaluated by enzyme-linked immunosorbent assay (ELISA) and by the indirect immunoperoxidase technique on sections of frozen and fixed human kidney and other tissues. The distribution of amyloid P component in various normal tissues is described and the possible co-localization with the Goodpasture antigen is discussed. In addition, the suitability of the antibody for detection of amyloid deposits in renal amyloidosis is demonstrated and its potential for use in other pathological conditions is considered. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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