Applicability of oral fluid collected onto filter paper for detection and genetic characterization of measles virus strains
| Autor: | Mike G. Catton, Chris Birch, Doris Chibo, Michaela A Riddell |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
biology Paramyxoviridae Reverse Transcriptase Polymerase Chain Reaction Context (language use) biology.organism_classification medicine.disease Virology Measles Virus Microbiology Specimen Handling Measles virus Morbillivirus medicine Humans RNA Viral Mononegavirales Saliva Genotyping Filtration |
| Zdroj: | Journal of clinical microbiology. 43(7) |
| ISSN: | 0095-1137 |
| Popis: | Expansion of measles molecular surveillance to developing countries where measles is endemic will help facilitate measles control. Limited infrastructure in these areas is a barrier to referral of specimens suitable for measles virus (MV) genotyping. In this study, we demonstrate that oral fluid dried onto filter paper can be used for the detection and characterization of MV strains. Using this approach, an MV-positive sample by reverse transcriptase PCR could be obtained from 67% of serologically confirmed acute measles cases. Mimicking certain environmental conditions and duration of transportation established that MV RNA remained detectable and suitable for nucleic acid sequencing in oral fluid spots for at least 1 week. In the context of a measles outbreak in a remote region of the world where infrastructure is poor, oral fluid samples dried onto filter paper and sent to a specialized laboratory for testing will aid in the identification and characterization of the causative MV strain. |
| Databáze: | OpenAIRE |
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