On the application of Job's method of continuous variation to the stoichiometry of protein-ligand complexes

Autor:	K.C. Ingham
Rok vydání:	1975
Předmět:	Binding Sites Ligand Chemistry Kinetics Biophysics Analytical chemistry Proteins Cell Biology Ligands Biochemistry Dissociation constant Methods Physical chemistry Binding site Molecular Biology Mathematics Stoichiometry Protein Binding Protein ligand
Zdroj:	Analytical Biochemistry. 68:660-663
ISSN:	0003-2697
DOI:	10.1016/0003-2697(75)90666-1
Popis:	The application of the method of continuous variation to protein-ligand complexes is critically examined for the case of a nonassociating protein which has n equivalent and independent ligand-binding sites. It is shown that in order to obtain a reliable estimate of the stoichiometry, the sum of protein and ligand concentrations must be kept large relative to the dissociation constant.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::399d7fd801016cb2d483c68a6b89c38e https://doi.org/10.1016/0003-2697(75)90666-1 Zobrazit plný text záznamu