Platelets express and release osteocalcin and co‐localize in human calcified atherosclerotic plaques
| Autor: | Giacomo Strapazzon, Andrea Garolla, F. Grego, Silvia Vettore, Carlo Foresta, Fabrizio Fabris, Gino Gerosa, L. De Toni |
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| Rok vydání: | 2013 |
| Předmět: |
Platelets
Blood Platelets Carotid Artery Diseases Male Platelet Membrane Glycoprotein IIb Pathology medicine.medical_specialty Myeloid Blotting Western Osteocalcin Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Inflammation Endarterectomy Cell Separation Biology Megakaryocyte CD63 medicine Humans Platelet Platelet activation Antigens Vascular Calcification Carotid Plaque Atherosclerotic Endarterectomy Carotid Blotting Tetraspanin 30 Secretory Vesicles Hematology Atherosclerosis Flow Cytometry Platelet Activation medicine.disease Plaque Atherosclerotic medicine.anatomical_structure Case-Control Studies Hemostasis Vascular calcification Antigens CD63 Megakaryocytes biology.protein medicine.symptom Western Calcification |
| Zdroj: | Journal of Thrombosis and Haemostasis. 11:357-365 |
| ISSN: | 1538-7836 |
| DOI: | 10.1111/jth.12088 |
| Popis: | Summary. Background: Although vascular-calcification mechanisms are only partially understood, the role of circulating calcifying cells and non-collagenous bone matrix proteins in the bone–vascular axis is emerging. In spite of the fact that platelets represent a cellular interface between hemostasis, inflammation and atherosclerosis, and have a myeloid precursor, a possible involvement in the modulation of vascular calcification has rarely been investigated. We investigated if osteocalcin (OC) is released by platelets and described OC expression in patients with carotid artery occlusive disease. Methods: Expression and release of OC were determined by Western blot, immunofluorescence, fluorescence-activated cell sorting (FACS) and ELISA in human resting and activated platelets and megakaryocytes. Co-localization of platelet aggregates, macrophages, OC and calcifications was studied in carotid endarterectomy specimens and normal tissues. Results: Human platelets expressed OC and co-localized with CD63 in δ-granules. Upon activation with an endogenous mechanism, platelets released OC in the extracellular medium. Expression of OC in megakaryocytes suggested lineage specificity. The OC count in circulating platelets and the released amount were significantly higher in patients with carotid artery occlusive disease than in healthy controls (P |
| Databáze: | OpenAIRE |
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