Assessing the performance of quantity and quality metrics using the QIAGEN Investigator® Quantiplex® pro RGQ kit
| Autor: | Linus Girdland-Flink, Suzzanne M. McColl, Jari Louhelainen, Jack Morrison, Kayleigh Sheppard, Giles D. J. Watts, Nick Dawnay, Ashley May |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Biology 01 natural sciences Pathology and Forensic Medicine QH301 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine DNA degradation Humans Multiplex 030216 legal & forensic medicine QH426 Sexual assault Chromatography 010401 analytical chemistry Significant difference DNA DNA Fingerprinting 0104 chemical sciences Benchmarking Real-time polymerase chain reaction Ancient DNA STR analysis chemistry Female Microsatellite Repeats |
| Zdroj: | Science & Justice. 60:388-397 |
| ISSN: | 1355-0306 |
| DOI: | 10.1016/j.scijus.2020.03.002 |
| Popis: | The Quantiplex® Pro RGQ kit quantifies DNA in a sample, supports the detection of mixtures and assesses the extent of DNA degradation based on relative ratios of amplified autosomal and male markers. Data show no significant difference in the accuracy and sensitivity of quantification between this and the Promega PowerQuant® System, both detecting the lowest amount of DNA tested, 4 pg. Laboratory controlled mixed male:female DNA samples together with mock sexual assault samples were quantified across a range of mixture ratios. Analysis software detected mixed DNA samples across all ratios for both quantification kits. Subsequent STR analysis using the Investigator® 24Plex QS Kit was able to corroborate mixture detection down to 1:25 male:female DNA ratios, past which point mixtures appeared identical to single-source female samples. Analysis software also detected laboratory degraded DNA samples, with data showing a positive trend between the Degradation Index (DI) and length of time of sonication. When used on ancient remains the assay was able to triage samples for further analysis, and STR profiles were concordant with DNA quantification results in all instances. STR analyses of laboratory-controlled sensitivity, mixture, and degradation studies supports the quality metric obtained from quantification. These data support the use of the Quantiplex® Pro RGQ kit for sample screening and quantification in forensic casework and ancient DNA studies. |
| Databáze: | OpenAIRE |
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