NdhO, a Subunit of NADPH Dehydrogenase, Destabilizes Medium Size Complex of the Enzyme in Synechocystis sp. Strain PCC 6803
| Autor: | Fudan Gao, Jingsong Zhang, Teruo Ogawa, Jiaohong Zhao, Weimin Ma |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Protein subunit
Mutant Bioenergetics Photosystem I Biochemistry Electron Transport Bacterial Proteins Enzyme Stability Molecular Biology chemistry.chemical_classification NADPH dehydrogenase Photosystem I Protein Complex biology Strain (chemistry) Synechocystis NADH dehydrogenase NADH Dehydrogenase Cell Biology biology.organism_classification Molecular biology Cell biology Protein Subunits Enzyme chemistry biology.protein Gene Deletion |
| Zdroj: | Journal of Biological Chemistry. 289:26669-26676 |
| ISSN: | 0021-9258 |
| Popis: | Two mutants that grew faster than the wild-type (WT) strain under high light conditions were isolated from Synechocystis sp. strain PCC 6803 transformed with a transposon-bearing library. Both mutants had a tag in ssl1690 encoding NdhO. Deletion of ndhO increased the activity of NADPH dehydrogenase (NDH-1)-dependent cyclic electron transport around photosystem I (NDH-CET), while overexpression decreased the activity. Although deletion and overexpression of ndhO did not have significant effects on the amount of other subunits such as NdhH, NdhI, NdhK, and NdhM in the cells, the amount of these subunits in the medium size NDH-1 (NDH-1M) complex was higher in the ndhO-deletion mutant and much lower in the overexpression strain than in the WT. NdhO strongly interacts with NdhI and NdhK but not with other subunits. NdhI interacts with NdhK and the interaction was blocked by NdhO. The blocking may destabilize the NDH-1M complex and repress the NDH-CET activity. When cells were transferred from growth light to high light, the amounts of NdhI and NdhK increased without significant change in the amount of NdhO, thus decreasing the relative amount of NdhO. This might have decreased the blocking, thereby stabilizing the NDH-1M complex and increasing the NDH-CET activity under high light conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|