Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent
| Autor: | Minna Juvonen, Sun-Li Chong, Maija Tenkanen, Ewa J. Mellerowicz, Marta Derba-Maceluch, Sanna Koutaniemi |
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| Rok vydání: | 2015 |
| Předmět: |
Pentoses
Arabidopsis Oligosaccharides Pentose Tandem mass spectrometry Mass spectrometry Biochemistry Glucuronic Acid Gene Expression Regulation Plant Structural Biology Glucuronoxylan Arabidopsis thaliana Pentosyltransferases Molecular Biology chemistry.chemical_classification Endo-1 4-beta Xylanases biology Arabidopsis Proteins General Medicine Oligosaccharide biology.organism_classification chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Xylans |
| Zdroj: | International Journal of Biological Macromolecules. 79:807-812 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2015.05.056 |
| Popis: | Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls. |
| Databáze: | OpenAIRE |
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