Perinatal DEHP exposure modulates pituitary estrogen receptor α and β expression altering lactotroph and somatotroph cell growth in prepuberal and adult male rats
| Autor: | Alicia Inés Torres, Pablo Anibal Pérez, Ana L. De Paul, Florencia Picech, Jorge Humberto Mukdsi, Juan Pablo Petiti, Graciela Díaz-Torga, Jonathan Toledo, Silvina Gutiérrez |
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| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Somatotropic cell Lactotrophs Estrogen receptor Endocrine Disruptors Toxicology Prolactin cell chemistry.chemical_compound Pregnancy Diethylhexyl Phthalate Internal medicine medicine Animals Endocrine system Rats Wistar Somatotroph Cell Cell Proliferation Fetus Cell growth Phthalate General Medicine Somatotrophs Rats Endocrinology Receptors Estrogen chemistry Pituitary Gland Prenatal Exposure Delayed Effects Female Food Science |
| Zdroj: | Food and Chemical Toxicology. 158:112649 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/j.fct.2021.112649 |
| Popis: | Phthalates are synthetic chemicals widely used to make polyvinylchloride (PVC) soft and flexible. Of these, Di-(2-ethylhexyl) phthalate (DEHP) is the most commonly used, with high human exposure occurring as early as the fetal developmental stage and affecting the endocrine system. We focused on the perinatal DEHP effects on pituitary estrogen receptor (ER) expression in male rats, explored their impact on lactotroph and somatotroph cell growth, and evaluated the direct effects of this phthalate on pituitary cell cultures. Our results showed that DEHP perinatal exposure was unable to modify the ERα+ pituitary cell number from prepuberal rats, but increased ERβ+ cells. In adulthood, the pituitary ERα+ cells underwent a slight decrease with ERβ showing the greatest changes, and with a significant increase observed in somatotroph cells. Also, in vitro, DEHP reduced the ERα+ cells, increased the percentage of ERβ+ pituitary cells and modified the Ki67 index, as well as decreasing the lactotrophs and increasing the somatotroph cells. In conclusion, the present study showed that DEHP induced ER expression changes in normal pituitary glands from male rats in in vivo and in vitro conditions, suggesting that DEHP could differentially modulate lactotroph and somatotroph cell growth, possibly as a consequence of ER imbalance. |
| Databáze: | OpenAIRE |
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