Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation

Autor: Xiangning Deng, Youyi Zhang, Han Xiao, Wei Gao, Juan Gao, Xinyu Wang, Jimin Wu
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Bodily Secretions
animal diseases
medicine.medical_treatment
Autophagy-Related Protein 5
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
Myocytes
Cardiac
Creatine Kinase
General Environmental Science
Aged
80 and over
Middle Aged
respiratory system
Troponin
Cytokine
030220 oncology & carcinogenesis
General Agricultural and Biological Sciences
Signal Transduction
Adult
medicine.medical_specialty
Adolescent
ATG5
Ischemia
Myocardial Reperfusion Injury
chemical and pharmacologic phenomena
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Internal medicine
Lactate dehydrogenase
Autophagy
otorhinolaryngologic diseases
medicine
Animals
Humans
Secretion
Acute Coronary Syndrome
Macrophage Migration-Inhibitory Factors
Cell damage
Aged
L-Lactate Dehydrogenase
business.industry
Fibroblasts
medicine.disease
biological factors
Rats
030104 developmental biology
Endocrinology
chemistry
Macrophage migration inhibitory factor
business
Biomarkers
Zdroj: Science China Life Sciences. 62:1038-1046
ISSN: 1869-1889
1674-7305
DOI: 10.1007/s11427-019-9567-1
Popis: Macrophage migration inhibitory factor (MIF) is an inflammatory cytokine. It is elevated early in the blood of acute myocardial infarction patients. However, it is unclear whether and how MIF is released. This study investigated the cellular source and mechanism of MIF release from hearts. An ischemia-mimic treatment induced the secretion of MIF from neonatal rat cardiomyocytes but not from fibroblasts. The treatment did not cause significant leakage of lactate dehydrogenase, suggesting that ischemia induced the MIF secretion without causing severe cell damage. Plasma samples from patients with acute chest pain at the emergency department were collected for the detection of MIF. MIF levels in patients with acute coronary syndrome (ACS) increased early, when cardiac injury markers were not yet elevated, suggesting that ischemia can induce MIF secretion before the occurrence of severe myocardial damage. Serum-starvation caused MIF secretion from rat cardiomyocytes and Langendorff-perfused rat hearts. The secretion was suppressed by the inhibition of autophagy by inhibitors or by silencing of Atg5. In conclusion, serum-starvation induces the secretion of MIF from cardiomyocytes via autophagy dependent pathway. Clarifying the mechanism of MIF secretion will be helpful for its application in the early diagnosis and treatment of ACS.
Databáze: OpenAIRE
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