Distribution and phylogeny of Wolbachia strains in wild mosquito populations in Sri Lanka
| Autor: | S. H. P. Parakrama Karunaratne, W. A. Priyanka P. de Silva, N. W. Nalaka P. Nugapola |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aedes albopictus Culex Mosquito Vectors lcsh:Infectious and parasitic diseases 03 medical and health sciences Aedes Anopheles parasitic diseases Animals lcsh:RC109-216 reproductive and urinary physiology Phylogeny Sri Lanka biology Research fungi Chaperonin 60 biochemical phenomena metabolism and nutrition Mosquito control biology.organism_classification Virology Wolbachia strains Culex quinquefasciatus Wsp Culicidae 030104 developmental biology Infectious Diseases Biological control Mansonia uniformis bacteria Parasitology Wolbachia Bacterial Outer Membrane Proteins |
| Zdroj: | Parasites & Vectors, Vol 10, Iss 1, Pp 1-8 (2017) Parasites & Vectors |
| ISSN: | 1756-3305 |
| Popis: | Wolbachia are a group of maternally inherited intracellular bacteria known to be widespread among arthropods. Infections with Wolbachia cause declines of host populations, and also induce host resistance to a wide range of pathogens. Over the past few decades, researchers were curious to use Wolbachia as a biological tool to control mosquito vectors. During the present study, assessment of the prevalence of Wolbachia infections among wild mosquito populations in Sri Lanka where mosquito-borne diseases are a major health concern, was carried out for the first time. DNA was extracted from the abdomens of mosquitoes, collected from seven provinces, and screened for the presence of Wolbachia by PCR using wsp and groE primers. Group-specific and strain-specific primers were used to classify Wolbachia into the supergroups A and B, and into the strains Mel, AlbA and Pip. A total of 330 individual mosquitoes belonging to 22 species and 7 genera were screened. Eighty-seven mosquitoes (26.36%) belonging to four species (i.e. Aedes albopictus, Culex quinquefasciatus, Armigeres subalbatus and Mansonia uniformis) were positive for Wolbachia infections. Primary vector of the dengue fever, Ae. aegypti was negative for Wolbachia infections while the secondary vector, Ae. albopictus, showed a very high infection rate. The filarial vector C. quinquefasciatus had a relatively high rate of infection. Japanese encephalitis vectors C. gelidus and C. triteaneorynchus, and the Anopheles vectors of malaria were negative for Wolbachia infections. Nine sequences of Wolbachia-positive PCR products were deposited in the GenBank and compared with other available data. Aedes albopictus was infected with both Wolbachia strains A (AlbA) and B (Pip) supergroups. Phylogenetic analysis of the wsp sequences showed two major branches confirming identities obtained from the PCR screening with strain-specific primers. Wolbachia infections were found only among four mosquito species in Sri Lanka: Aedes albopictus, Culex quinquefasciatus, Armigeres subalbatus and Mansonia uniformis. Sequence data showed high haplotype diversity among the Wolbachia strains. |
| Databáze: | OpenAIRE |
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