Molecular identification and antifungal susceptibilities of black Aspergillus isolates from otomycosis cases in Hungary
| Autor: | Ilona Dóczi, László Bereczki, Csaba Vágvölgyi, Sándor Kocsubé, János Varga, Gyöngyi Szigeti |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Antifungal Agents Adolescent Itraconazole Veterinary (miscellaneous) Ear infection Molecular Sequence Data Otomycosis Microbial Sensitivity Tests Applied Microbiology and Biotechnology Microbiology Fungal Proteins Calmodulin Amphotericin B medicine Aspergillosis Humans DNA Fungal Aged Aged 80 and over Aspergillus Hungary biology Aspergillus niger Fungal genetics Pigments Biological Sequence Analysis DNA Middle Aged biology.organism_classification medicine.disease Terbinafine Female Agronomy and Crop Science medicine.drug |
| Zdroj: | Mycopathologia. 174(2) |
| ISSN: | 1573-0832 |
| Popis: | Otomycosis, also known as fungal otitis externa, has been used to describe a fungal infection of the external auditory canal, but sometimes involving the middle ear. Many fungal species have been identified as infectious agents in otomycosis, with Aspergillus and Candida species being the most common. Among aspergilli, Aspergillus niger is the most commonly described species in the literature. In this study, 14 black Aspergillus strains were analyzed, which were isolated from otomycosis cases in Hungary between 2010 and 2011. These strains were identified as A. niger according to conventional morphological methods. Species identification was based on sequencing of part of the calmodulin gene. Our results indicate that instead of A. niger, A. awamori and A. tubingensis are the predominant species that cause ear infections in Southern Hungary. Antifungal susceptibility tests were carried out against four antifungal drugs: amphotericin B, itraconazole, ketoconazole and terbinafine. All isolates were found to exhibit low in vitro MIC values to amphotericin B, terbinafine and itraconazole. However, the examined isolates exhibited high in vitro MIC values to ketoconazole. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink