Antibodies targeting linear determinants of the envelope protein protect mice against West Nile virus
| Autor: | Nathalie Bonafé, Kalipada Kar, Karen Anthony, Sandra L. Bushmich, Raymond A. Koski, Harald G. Foellmer, L. Hannah Gould, Michel Ledizet, Erol Fikrig |
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| Rok vydání: | 2008 |
| Předmět: |
viruses
Molecular Sequence Data Antibody Affinity Fluorescent Antibody Technique Immunoglobulins Antibodies Viral Epitope Virus law.invention Flaviviridae Epitopes Mice Affinity chromatography Viral Envelope Proteins law Chlorocebus aethiops Immunology and Allergy Animals Flavivirus Infections Amino Acid Sequence Horses Vero Cells Mice Inbred C3H biology Immunization Passive virus diseases biology.organism_classification Virology Peptide Fragments Flavivirus Infectious Diseases biology.protein Recombinant DNA Drosophila Female Antibody West Nile virus West Nile Fever |
| Zdroj: | The Journal of infectious diseases. 196(12) |
| ISSN: | 0022-1899 |
| Popis: | The flavivirus envelope (E) protein mediates cellular attachment and fusion with host cell membranes and is recognized by virus-neutralizing antibodies. We raised antibodies against a broad range of epitopes by immunizing a horse with recombinant West Nile virus (WNV) E protein. To define epitopes recognized by protective antibodies, we selected, by affinity chromatography, immunoglobulins against immobilized linear peptides derived from parts of the E protein. Immunoglobulins binding 9 different peptides from domains I, II, and III of the E protein neutralized WNV in vitro. This indicates that multiple protective epitopes can be found in the E protein. Immunoglobulins recognizing 3 peptides derived from domains I and II of E protein protected mice against a lethal challenge with WNV. These immunoglobulins recognized the E proteins of related flaviviruses, demonstrating that antibodies targeting specific E protein epitopes could be developed for prevention and treatment of multiple flavivirus infections. |
| Databáze: | OpenAIRE |
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