Glycoglycerolipid from the Membranes of Acholeplasma laidlawii Binds to Human Immunodeficiency Virus-1 (HIV-1) and Accelerates Its Entry into Cells
| Autor: | Koichi Kuwano, Takashi Shimizu, Yutaka Kida, Atsushi Koito, Sumio Arai, Masahiro Hirama, Hiroto Imai, Tohru Oishi |
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| Rok vydání: | 2004 |
| Předmět: |
T-Lymphocytes
HIV Core Protein p24 Human immunodeficiency virus (HIV) Glycoglycerolipid Biology Virus Replication medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Cell Line Glycolipid Acholeplasma laidlawii medicine Humans Immune Sera Ligand binding assay General Medicine biology.organism_classification Membrane Viral replication Biochemistry Cell culture HIV-1 lipids (amino acids peptides and proteins) Glycolipids |
| Zdroj: | Current Microbiology. 48:182-188 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-003-4101-x |
| Popis: | We have reported previously that glycoglycerolipids derived from the membranes of Acholeplasma laidlawii, 3- O-[2'- O-(alpha-D-glucopyranosyl)-6'- O-acyl-alpha-glucopyranosyl]-1,2-di- O-acyl- sn-glycerols (GAGDGs) bind to human cell lines. In addition, the GAGDGs were found to augment HIV-1 infection in human cell lines. Here we show that GAGDG binds to HIV-1 and facilitates the entry of HIV-1 into cells. The binding ability of GAGDG to HIV-1 was blocked by anti-GAGDG serum. Binding assay with synthetic GAGDGs and related compounds showed that the presence of branching form of acyl chains at the C14 or C16 position, glucose, and the acyl chain binding to the glucose were critical for efficient binding. GAGDG efficiently augmented the entry of HIV-1 into cells in a single-cycle replication assay. These results indicate that GAGDG of A. laidlawii membranes participates in the facilitation of HIV-1 infection. |
| Databáze: | OpenAIRE |
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