Levels of oxidative stress biomarkers and bone resorption regulators in apical periodontitis lesions infected by Epstein-Barr virus
| Autor: | T. Radosavljevic, Aleksandra Knezevic, Marija Pljesa-Ercegovac, Vladimir Ivanovic, Jelena Milasin, Tatjana Simic, Vesna Coric, Katarina Beljic-Ivanovic, Ivan Soldatovic, Jelena Carkic, Tanja Jovanovic, Nađa Nikolic, Aleksandar Jakovljevic, Miroslav Andric, S. Krezovic, Maja Miletic |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male musculoskeletal diseases 0301 basic medicine Epstein-Barr Virus Infections Herpesvirus 4 Human medicine.medical_specialty Enzyme-Linked Immunosorbent Assay Real-Time Polymerase Chain Reaction medicine.disease_cause Bone resorption 03 medical and health sciences 0302 clinical medicine Osteoprotegerin Internal medicine Epstein-Barr virus Humans Medicine Bone Resorption Receptor General Dentistry Periodontitis biology business.industry RANK Ligand Deoxyguanosine 030206 dentistry apical periodontitis medicine.disease Glutathione Epstein–Barr virus 3. Good health Oxidative Stress 030104 developmental biology Endocrinology 8-Hydroxy-2'-Deoxyguanosine RANKL Case-Control Studies biology.protein Pulp (tooth) Female business Biomarkers Periapical Periodontitis Oxidative stress |
| Zdroj: | International Endodontic Journal |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/iej.12886 |
| Popis: | AIM To investigate whether apical periodontitis lesions infected by Epstein-Barr virus (EBV) exhibit higher levels of oxidative stress biomarkers [8-hydroxydeoxyguanosine (8-OHdG) and oxidized glutathione (GSSG)] and bone resorption regulators [receptor activator of nuclear factor (NF-κB) ligand (RANKL) and osteoprotegerin (OPG)] compared to EBV-negative periapical lesions and healthy pulp tissues. METHODOLOGY The experimental group consisted of 30 EBV-positive and 30 EBV-negative periapical lesions collected in conjunction with apicoectomy. The pulp tissues of 20 impacted third molars were used as healthy controls. The qualitative and quantitative analysis of EBV was performed by nested and real-time polymerase chain reaction (PCR), respectively. The levels of RANKL and OPG were analysed by reverse transcriptase real-time PCR. The levels of 8-OHdG and GSSG were determined by enzyme-linked immunosorbent assay (ELISA). Mann-Whitney U-test and Spearman's correlation were used for statistical analysis. RESULTS The levels of RANKL, OPG, 8-OHdG and GSSG were significantly higher in apical periodontitis lesions compared to healthy pulp controls (P = 0.001, P |
| Databáze: | OpenAIRE |
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