Tumour lineage-homing cell-penetrating peptides as anticancer molecular delivery systems
| Autor: | Eisaku Kondo, Naoya Kobayashi, Ken Saito, Kaeko Kamide, Shusei Uno, Masahiro Nishibori, Masao Mashita, Yuichi Tashiro, Tomoyuki Tsumuraya, Kiichiro Nakajima, Mitsune Tanimoto, Masayuki Matsushita, Tomoko Furuya |
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| Rok vydání: | 2012 |
| Předmět: |
Immunoblotting
Cell General Physics and Astronomy Antineoplastic Agents Cell-Penetrating Peptides Pharmacology Biology General Biochemistry Genetics and Molecular Biology Cell Line Mice Drug Delivery Systems Peptide Library Cell Line Tumor Neoplasms medicine Animals Humans RNA Small Interfering Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction fungi food and beverages General Chemistry Surface Plasmon Resonance Flow Cytometry Xenograft Model Antitumor Assays Cell biology medicine.anatomical_structure Nucleic acid Homing (hematopoietic) |
| Zdroj: | Nature Communications. 3 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms1952 |
| Popis: | Cell-penetrating peptides have gained attention owing to their promise in noninvasive delivery systems. Among the identified cell-penetrating peptides, the TAT peptide has been preferentially used for transduction into cells of diverse origins. However, this activity is nonselective between neoplastic and non-neoplastic cells. Here we describe artificial cell-penetrating peptides that are selectively and efficiently incorporated into human tumour cells, according to their lineage. Ten representative tumour lineage-homing cell-penetrating peptides were obtained by screening of a random peptide library constructed using messenger RNA display technology, and some of the isolates were further modified by amino-acid substitution. Their advantageous tumour cell-targeting ability is corroborated in an in vivo mouse model for imaging and growth suppression of metastatic xenoplant tumours. These cell-penetrating peptides are potentially useful for the efficient targeting of human neoplasms in a tumour origin-dependent manner, and provide a framework for the development of peptide-based anti-tumour technologies. |
| Databáze: | OpenAIRE |
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